Heterologous expression of the fungal polyketide bikaverin and effects of PPTase co-expression

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

Abstrakt

The biosynthetic pathway of the Fusarium verticillioides polyketide bikaverin (PKS16) was reconstructed
utilizing a plasmid-based system and heterologously expressed in Saccharomyces cerevisiae BY4743.
Through NGS-sequencing and subsequent HPLC MS-MS analysis of chemical extracts, the reconstruction of
the biosynthetic pathway was validated and the red pigment of bikaverin was produced. While
heterologous expressing fungal polyketides in S. cerevisiae the co-expression of a helper enzyme, a 4-
phosphopantetheinyltransferase (PPTase), is necessary for transforming the ACP-domain of the polyketide
synthase (PKS) from an inactive apo-conformation to the active holo-conformation. This study elucidates
the effect of seven different PPTases on the overall yield of bikaverin and determines the most efficient to
be FvPPT, a PPTase also originating from Fusarium verticillioides, yielding 58 mg/L bikaverin. This could
indicate an evolutionary advantage when pairing PPTases and pathways sharing the same origin, in
heterologous expression systems
OriginalsprogEngelsk
Publikationsdato14 okt. 2019
StatusUdgivet - 14 okt. 2019
BegivenhedSBA2019: Synthetic Biology Australasia Conference 2019 - The Old Customs House, Brisbane, Australien
Varighed: 14 okt. 201916 okt. 2019
https://synbioaustralasia.org/2019-sba-conference/

Konference

KonferenceSBA2019
LokationThe Old Customs House
LandAustralien
ByBrisbane
Periode14/10/201916/10/2019
Internetadresse

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