Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum

Jens Laurids Sørensen; Teis Esben Sondergaard; Lorenzo Covarelli; Patricia Romans Fuertes; Frederik Teilfeldt Hansen; Rasmus John Normand Frandsen; Wagma Saei; Mie Bech Lukassen; Reinhard Wimmer; Kristian Fog Nielsen; Donald Max Gardiner; Henriette Giese

doi:10.1021/np500436r

Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum

Jens Laurids Sørensen, Teis Esben Sondergaard, Lorenzo Covarelli, Patricia Romans Fuertes, Frederik Teilfeldt Hansen, Rasmus John Normand Frandsen, Wagma Saei, Mie Bech Lukassen, Reinhard Wimmer, Kristian Fog Nielsen, Donald Max Gardiner, Henriette Giese

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

49 Citationer (Scopus)

Abstract

The closely related species Fusarium graminearum and Fusarium pseudograminearum differ in that each contains a gene cluster with a polyketide synthase (PKS) and a nonribosomal peptide synthetase (NRPS) that is not present in the other species. To identify their products, we deleted PKS6 and NRPS7 in F. graminearum and NRPS32 in F. pseudograminearum. By comparing the secondary metabolite profiles of the strains we identified the resulting product in F. graminearum as fusaristatin A, and as W493 A and B in F. pseudograminearum. These lipopeptides have previously been isolated from unidentified Fusarium species. On the basis of genes in the putative gene clusters we propose a model for biosynthesis where the polyketide product is shuttled to the NPRS via a CoA ligase and a thioesterase in F. pseudograminearum. In F. graminearum the polyketide is proposed to be directly assimilated by the NRPS.

Originalsprog	Engelsk
Tidsskrift	Journal of Natural Products
Vol/bind	77
Udgave nummer	12
Sider (fra-til)	2619-2625
Antal sider	7
ISSN	0163-3864
DOI	https://doi.org/10.1021/np500436r
Status	Udgivet - 26 dec. 2014

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Publisher Copyright:
© 2014 The American Chemical Society and American Society of Pharmacognosy.

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Sørensen, J. L., Sondergaard, T. E., Covarelli, L., Fuertes, P. R., Hansen, F. T., Frandsen, R. J. N., Saei, W., Lukassen, M. B., Wimmer, R., Nielsen, K. F., Gardiner, D. M., & Giese, H. (2014). Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum. Journal of Natural Products, 77(12), 2619-2625. https://doi.org/10.1021/np500436r

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title = "Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum",

abstract = "The closely related species Fusarium graminearum and Fusarium pseudograminearum differ in that each contains a gene cluster with a polyketide synthase (PKS) and a nonribosomal peptide synthetase (NRPS) that is not present in the other species. To identify their products, we deleted PKS6 and NRPS7 in F. graminearum and NRPS32 in F. pseudograminearum. By comparing the secondary metabolite profiles of the strains we identified the resulting product in F. graminearum as fusaristatin A, and as W493 A and B in F. pseudograminearum. These lipopeptides have previously been isolated from unidentified Fusarium species. On the basis of genes in the putative gene clusters we propose a model for biosynthesis where the polyketide product is shuttled to the NPRS via a CoA ligase and a thioesterase in F. pseudograminearum. In F. graminearum the polyketide is proposed to be directly assimilated by the NRPS.",

author = "S{\o}rensen, {Jens Laurids} and Sondergaard, {Teis Esben} and Lorenzo Covarelli and Fuertes, {Patricia Romans} and Hansen, {Frederik Teilfeldt} and Frandsen, {Rasmus John Normand} and Wagma Saei and Lukassen, {Mie Bech} and Reinhard Wimmer and Nielsen, {Kristian Fog} and Gardiner, {Donald Max} and Henriette Giese",

note = "Publisher Copyright: {\textcopyright} 2014 The American Chemical Society and American Society of Pharmacognosy.",

year = "2014",

month = dec,

day = "26",

doi = "10.1021/np500436r",

language = "English",

volume = "77",

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Sørensen, JL , Sondergaard, TE, Covarelli, L, Fuertes, PR, Hansen, FT, Frandsen, RJN, Saei, W , Lukassen, MB , Wimmer, R, Nielsen, KF, Gardiner, DM & Giese, H 2014, 'Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum', Journal of Natural Products, bind 77, nr. 12, s. 2619-2625. https://doi.org/10.1021/np500436r

Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum. / Sørensen, Jens Laurids ; Sondergaard, Teis Esben; Covarelli, Lorenzo et al.
I: Journal of Natural Products, Bind 77, Nr. 12, 26.12.2014, s. 2619-2625.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum

AU - Sørensen, Jens Laurids

AU - Sondergaard, Teis Esben

AU - Covarelli, Lorenzo

AU - Fuertes, Patricia Romans

AU - Hansen, Frederik Teilfeldt

AU - Frandsen, Rasmus John Normand

AU - Saei, Wagma

AU - Lukassen, Mie Bech

AU - Wimmer, Reinhard

AU - Nielsen, Kristian Fog

AU - Gardiner, Donald Max

AU - Giese, Henriette

PY - 2014/12/26

Y1 - 2014/12/26

N2 - The closely related species Fusarium graminearum and Fusarium pseudograminearum differ in that each contains a gene cluster with a polyketide synthase (PKS) and a nonribosomal peptide synthetase (NRPS) that is not present in the other species. To identify their products, we deleted PKS6 and NRPS7 in F. graminearum and NRPS32 in F. pseudograminearum. By comparing the secondary metabolite profiles of the strains we identified the resulting product in F. graminearum as fusaristatin A, and as W493 A and B in F. pseudograminearum. These lipopeptides have previously been isolated from unidentified Fusarium species. On the basis of genes in the putative gene clusters we propose a model for biosynthesis where the polyketide product is shuttled to the NPRS via a CoA ligase and a thioesterase in F. pseudograminearum. In F. graminearum the polyketide is proposed to be directly assimilated by the NRPS.

AB - The closely related species Fusarium graminearum and Fusarium pseudograminearum differ in that each contains a gene cluster with a polyketide synthase (PKS) and a nonribosomal peptide synthetase (NRPS) that is not present in the other species. To identify their products, we deleted PKS6 and NRPS7 in F. graminearum and NRPS32 in F. pseudograminearum. By comparing the secondary metabolite profiles of the strains we identified the resulting product in F. graminearum as fusaristatin A, and as W493 A and B in F. pseudograminearum. These lipopeptides have previously been isolated from unidentified Fusarium species. On the basis of genes in the putative gene clusters we propose a model for biosynthesis where the polyketide product is shuttled to the NPRS via a CoA ligase and a thioesterase in F. pseudograminearum. In F. graminearum the polyketide is proposed to be directly assimilated by the NRPS.

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U2 - 10.1021/np500436r

DO - 10.1021/np500436r

M3 - Journal article

C2 - 25412204

SN - 0163-3864

VL - 77

SP - 2619

EP - 2625

JO - Journal of Natural Products

JF - Journal of Natural Products

IS - 12

ER -

Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum

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