Abstract
To study the role of Trichoderma in sick building syndrome, it is essential to be able to accurately identify species. Forty-four strains of Trichoderma spp. isolated from Danish buildings damaged by water leaks were identified using ITS1 ribotyping and universally primed PCR, UP-PCR. Ribotyping allowed the assignment of the strains into three distinct groups. High similarity of UP-PCR banding profiles of the strains allowed species designation for almost all strains (43 out of 44) when compared with the UP-PCR banding profiles obtained from reference strains of T. atroviride, T. citrinoviride, T. harzianum, T. longibrachiatum and T. viride. However, cross hybridization of UP-PCR products showed that the latter strain had high DNA homology to the ex-type strain of T. hamatum. The combined approach is a convenient way for reliable identification of Trichoderma strains. Copyright (C) 2000 Federation of European Microbiological Societies.
Originalsprog | Engelsk |
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Tidsskrift | FEMS Microbiology Letters |
Vol/bind | 185 |
Udgave nummer | 2 |
Sider (fra-til) | 129-134 |
Antal sider | 6 |
ISSN | 0378-1097 |
DOI | |
Status | Udgivet - 15 apr. 2000 |
Udgivet eksternt | Ja |