TY - JOUR
T1 - Immobilization of biomolecules onto surfaces according to ultraviolet light diffraction patterns
AU - Petersen, Steffen B.
AU - Gennaro, Ane Kold Di
AU - Neves Petersen, Teresa
AU - Skovsen, Esben
AU - Parracino, Antonietta
PY - 2010
Y1 - 2010
N2 - We developed a method for immobilization of biomolecules onto thiol functionalized surfaces according to UV diffraction patterns. UV light-assisted molecular immobilization proceeds through the formation of free, reactive thiol groups that can bind covalently to thiol reactive surfaces. We demonstrate that, by shaping the pattern of the UV light used to induce molecular immobilization, one can control the pattern of immobilized molecules onto the surface. Using a single-aperture spatial mask, combined with the Fourier transforming property of a focusing lens, we show that submicrometer (0.7 mu m) resolved patterns of immobilized prostate-specific antigen biomolecules can be created. If a dual-aperture spatial mask is used, the results differ from the expected Fourier transform pattern of the mask. It appears as a superposition of two diffraction patterns produced by the two apertures, with a fine structured interference pattern superimposed. (C) 2010 Optical Society of America
AB - We developed a method for immobilization of biomolecules onto thiol functionalized surfaces according to UV diffraction patterns. UV light-assisted molecular immobilization proceeds through the formation of free, reactive thiol groups that can bind covalently to thiol reactive surfaces. We demonstrate that, by shaping the pattern of the UV light used to induce molecular immobilization, one can control the pattern of immobilized molecules onto the surface. Using a single-aperture spatial mask, combined with the Fourier transforming property of a focusing lens, we show that submicrometer (0.7 mu m) resolved patterns of immobilized prostate-specific antigen biomolecules can be created. If a dual-aperture spatial mask is used, the results differ from the expected Fourier transform pattern of the mask. It appears as a superposition of two diffraction patterns produced by the two apertures, with a fine structured interference pattern superimposed. (C) 2010 Optical Society of America
KW - Fourier transform
KW - diffraction pattern
KW - UV light assisted molecular immobilisation
KW - high density biosensors
KW - arrays
U2 - 10.1364/AO.49.005344
DO - 10.1364/AO.49.005344
M3 - Journal article
SN - 0003-6935
VL - 49
SP - 5344
EP - 5350
JO - Applied Optics
JF - Applied Optics
IS - 28
ER -