TY - JOUR
T1 - Impact of the restraint of biofilm volume and thickness on the performance and microbial composition in anaerobic moving-bed biofilm reactors (AnMBBRs)
AU - Hermansson, Astrid
AU - Jacobsson, Susanne
AU - de Jonge, Nadieh
AU - Nielsen, Jeppe Lund
AU - Morgan-Sagastume, Fernando
N1 - Publisher Copyright:
© 2022 Elsevier Ltd. All rights reserved.
PY - 2022/6
Y1 - 2022/6
N2 - The effect of limiting biofilm volume using carriers with maximum thickness control on the performance and microbial community composition was investigated in anaerobic moving-bed biofilm reactors (AnMBBRs). Three parallel, continuous AnMBBRs (4 L) were operated (288 d, 24/36 °C) to treat the soluble fraction of a wastewater from a food-manufacturing facility. Two different biofilm carriers controlling maximum biofilm thickness at 200 and 1000 μm (AnoxK™Z-200 and Z-1000) were used in the three reactors, targeting the same projected surface area for biofilm growth (410 m2/reactor): R200 (Z-200), R1000 (Z-1000) and RMix (both Z-200 and Z-1000). The composition of the bacterial (16S rRNA V1-V3) and methanogenic (mcrA gene) communities was analysed using amplicon sequencing. Under relatively constant organic loads (3.6-4.2 gSCOD/Ld, 34-39 gSCOD/m2d, hydraulic retention time=7.7-8 h), R200 presented lower soluble chemical oxygen demand (SCOD) removals (51-60%) than the other reactors (R100 = 91-93%, RMix = 86-91%), likely due to a limited amount of active protected biofilm volume. Lower SCOD removals were associated with higher concentrations of volatile fatty acids in the effluent (R200 =400-800 mgCOD/L; R1000 and RMix<200 mgCOD/L) and lower methane production (R200 =0.66; R1000 =0.72; RMix=0.73 gCH4-COD/gSCODremoved). The Z-200 carriers contained a different fermentative/acidogenic bacterial community abundant in representatives of the families Christensenellaceae, Anaerovoracaceae and Synergistaceae, and led to less amount of biofilm biomass growth, albeit more active than that of carriers allowing for thicker biofilms (Z-1000). In contrast, methanogenic populations were less sensitive to biofilm thickness restraint by these carriers. This study shows for the first time that limiting biofilm volume in MBBR carriers can impact the performance and bacterial community in AnMBBR biofilms.
AB - The effect of limiting biofilm volume using carriers with maximum thickness control on the performance and microbial community composition was investigated in anaerobic moving-bed biofilm reactors (AnMBBRs). Three parallel, continuous AnMBBRs (4 L) were operated (288 d, 24/36 °C) to treat the soluble fraction of a wastewater from a food-manufacturing facility. Two different biofilm carriers controlling maximum biofilm thickness at 200 and 1000 μm (AnoxK™Z-200 and Z-1000) were used in the three reactors, targeting the same projected surface area for biofilm growth (410 m2/reactor): R200 (Z-200), R1000 (Z-1000) and RMix (both Z-200 and Z-1000). The composition of the bacterial (16S rRNA V1-V3) and methanogenic (mcrA gene) communities was analysed using amplicon sequencing. Under relatively constant organic loads (3.6-4.2 gSCOD/Ld, 34-39 gSCOD/m2d, hydraulic retention time=7.7-8 h), R200 presented lower soluble chemical oxygen demand (SCOD) removals (51-60%) than the other reactors (R100 = 91-93%, RMix = 86-91%), likely due to a limited amount of active protected biofilm volume. Lower SCOD removals were associated with higher concentrations of volatile fatty acids in the effluent (R200 =400-800 mgCOD/L; R1000 and RMix<200 mgCOD/L) and lower methane production (R200 =0.66; R1000 =0.72; RMix=0.73 gCH4-COD/gSCODremoved). The Z-200 carriers contained a different fermentative/acidogenic bacterial community abundant in representatives of the families Christensenellaceae, Anaerovoracaceae and Synergistaceae, and led to less amount of biofilm biomass growth, albeit more active than that of carriers allowing for thicker biofilms (Z-1000). In contrast, methanogenic populations were less sensitive to biofilm thickness restraint by these carriers. This study shows for the first time that limiting biofilm volume in MBBR carriers can impact the performance and bacterial community in AnMBBR biofilms.
KW - Anaerobic treatment
KW - Biofilm
KW - Biomass
KW - Media
KW - Methanogens
KW - Wastewater
KW - Abbreviations AnMBBR Anaerobic moving-bed biofilm reactor
KW - PSA Projected surface area
KW - HRT Hydraulic retention time
KW - MBBR Moving-bed biofilm reactor
KW - VLR Volumetric loading rate
KW - COD Chemical oxygen demand
KW - SALR Surface are loading rate
KW - ASV Amplicon sequencing variant
KW - HDPE High-density polyethylene
KW - DNA Deoxyribonucleic acid
KW - TS and TSS Total solids and Total suspended solids
KW - NMDS Non-metric multi-dimensional scaling
KW - VFA Volatile fatty acid
KW - SCOD Soluble chemical oxygen demand
KW - VS and VSS Volatile solids and Volatile suspended solids
KW - EGSB Expanded granular sludge bed
KW - RNA Ribonucleic acid
KW - UASB Upflow anaerobic sludge blanket
UR - http://www.scopus.com/inward/record.url?scp=85131129157&partnerID=8YFLogxK
U2 - 10.1016/j.jece.2022.107741
DO - 10.1016/j.jece.2022.107741
M3 - Journal article
AN - SCOPUS:85131129157
SN - 2213-3437
VL - 10
JO - Journal of Environmental Chemical Engineering
JF - Journal of Environmental Chemical Engineering
IS - 3
M1 - 107741
ER -