In Situ Atomic Force Microscopy Studies of the Effect of Indolicidin on E.coli Cells

Hans Jakob Askou, Rasmus Neergaard Jakobsen, Peter Fojan

Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftFormidling

Resumé

E.coli cells were succesfully attached to both gelatin coated surfaces and polylactic acid honeycomb patterned mica surfaces as determined by in situ atomic force microscopy. The gelatin coated surfaces provided a softer support onto which the E.coli cells were capable of slightly submerging leading to a better adhesion compared to the harder surfaces consisting of polylactic acid polymer surfaces. After continuous scanning in liquid media, the E.coli cells remained rod shaped and smooth. Indolicidin, a 13-AA linear antimicrobial peptide, was injected in order to visualize the peptide-membrane interactions in real time. Instantly after the injection of the peptides, the bacterial membranes were observed to be distorted and seemed to melt proceeding as a function of time. In conclusion, these experiments proved that the E.coli cells were not ruptured as could be expected due to pore formation and disruption of the osmotic pressure. This indicates a possible intracellular target killing mechanism of indolicidin interacting with E.coli cells.
OriginalsprogEngelsk
TidsskriftJournal of Self-Assembly and Molecular Electronics
Vol/bind2
Udgave nummer1
ISSN2245-4551
StatusUdgivet - 2019

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Escherichia coli
Atomic force microscopy
Gelatin
Peptides
Membranes
Polymers
Adhesion
indolicidin
Scanning
Liquids
Experiments
poly(lactic acid)

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title = "In Situ Atomic Force Microscopy Studies of the Effect of Indolicidin on E.coli Cells",
abstract = "E.coli cells were succesfully attached to both gelatin coated surfaces and polylactic acid honeycomb patterned mica surfaces as determined by in situ atomic force microscopy. The gelatin coated surfaces provided a softer support onto which the E.coli cells were capable of slightly submerging leading to a better adhesion compared to the harder surfaces consisting of polylactic acid polymer surfaces. After continuous scanning in liquid media, the E.coli cells remained rod shaped and smooth. Indolicidin, a 13-AA linear antimicrobial peptide, was injected in order to visualize the peptide-membrane interactions in real time. Instantly after the injection of the peptides, the bacterial membranes were observed to be distorted and seemed to melt proceeding as a function of time. In conclusion, these experiments proved that the E.coli cells were not ruptured as could be expected due to pore formation and disruption of the osmotic pressure. This indicates a possible intracellular target killing mechanism of indolicidin interacting with E.coli cells.",
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In Situ Atomic Force Microscopy Studies of the Effect of Indolicidin on E.coli Cells. / Askou, Hans Jakob; Jakobsen, Rasmus Neergaard; Fojan, Peter.

I: Journal of Self-Assembly and Molecular Electronics, Bind 2, Nr. 1, 2019.

Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftFormidling

TY - ABST

T1 - In Situ Atomic Force Microscopy Studies of the Effect of Indolicidin on E.coli Cells

AU - Askou, Hans Jakob

AU - Jakobsen, Rasmus Neergaard

AU - Fojan, Peter

PY - 2019

Y1 - 2019

N2 - E.coli cells were succesfully attached to both gelatin coated surfaces and polylactic acid honeycomb patterned mica surfaces as determined by in situ atomic force microscopy. The gelatin coated surfaces provided a softer support onto which the E.coli cells were capable of slightly submerging leading to a better adhesion compared to the harder surfaces consisting of polylactic acid polymer surfaces. After continuous scanning in liquid media, the E.coli cells remained rod shaped and smooth. Indolicidin, a 13-AA linear antimicrobial peptide, was injected in order to visualize the peptide-membrane interactions in real time. Instantly after the injection of the peptides, the bacterial membranes were observed to be distorted and seemed to melt proceeding as a function of time. In conclusion, these experiments proved that the E.coli cells were not ruptured as could be expected due to pore formation and disruption of the osmotic pressure. This indicates a possible intracellular target killing mechanism of indolicidin interacting with E.coli cells.

AB - E.coli cells were succesfully attached to both gelatin coated surfaces and polylactic acid honeycomb patterned mica surfaces as determined by in situ atomic force microscopy. The gelatin coated surfaces provided a softer support onto which the E.coli cells were capable of slightly submerging leading to a better adhesion compared to the harder surfaces consisting of polylactic acid polymer surfaces. After continuous scanning in liquid media, the E.coli cells remained rod shaped and smooth. Indolicidin, a 13-AA linear antimicrobial peptide, was injected in order to visualize the peptide-membrane interactions in real time. Instantly after the injection of the peptides, the bacterial membranes were observed to be distorted and seemed to melt proceeding as a function of time. In conclusion, these experiments proved that the E.coli cells were not ruptured as could be expected due to pore formation and disruption of the osmotic pressure. This indicates a possible intracellular target killing mechanism of indolicidin interacting with E.coli cells.

UR - https://doi.org/10.13052/jsame2245-4551.6.002

M3 - Conference abstract in journal

VL - 2

JO - Journal of Self-Assembly and Molecular Electronics

JF - Journal of Self-Assembly and Molecular Electronics

SN - 2245-4551

IS - 1

ER -