Influence of carbon-dioxide tension and medium buffer concentration on medium pH and MIC values of erythromycin for Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 in a micro-aerobic atmosphere

J J Andreasen, B Nissen, S H Hartzen

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Abstract

The effect of an elevated carbon-dioxide tension on medium pH and MIC determination with erythromycin for Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) was investigated in a micro-aerobic atmosphere, using an agar dilution method. During 30 h of incubation in an atmosphere containing approximately 6% CO2, 5% O2 and 89% N2, pH of uninoculated, as well as of seeded, blood agar plates decreased from 7.4 to 6.8. MIC values of erythromycin were two dilution steps higher after micro-aerobic incubation than after aerobic incubation. A decrease in agar pH during micro-aerobic incubation was eliminated by increasing the phosphate buffer concentration in the medium. However, MIC values of erythromycin remained high.

OriginalsprogEngelsk
TidsskriftActa pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology
Vol/bind95
Udgave nummer4
Sider (fra-til)253-5
Antal sider3
ISSN0108-0180
StatusUdgivet - aug. 1987
Udgivet eksterntJa

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