Abstract
The effect of an elevated carbon-dioxide tension on medium pH and MIC determination with erythromycin for Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) was investigated in a micro-aerobic atmosphere, using an agar dilution method. During 30 h of incubation in an atmosphere containing approximately 6% CO2, 5% O2 and 89% N2, pH of uninoculated, as well as of seeded, blood agar plates decreased from 7.4 to 6.8. MIC values of erythromycin were two dilution steps higher after micro-aerobic incubation than after aerobic incubation. A decrease in agar pH during micro-aerobic incubation was eliminated by increasing the phosphate buffer concentration in the medium. However, MIC values of erythromycin remained high.
Originalsprog | Engelsk |
---|---|
Tidsskrift | Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology |
Vol/bind | 95 |
Udgave nummer | 4 |
Sider (fra-til) | 253-5 |
Antal sider | 3 |
ISSN | 0108-0180 |
Status | Udgivet - aug. 1987 |
Udgivet eksternt | Ja |