Abstract
Acid-base conditions modify artery tone and tissue perfusion but the involved vascular sensing mechanisms and disease consequences remain unclear. We experimentally investigated transgenic mice and performed genetic studies in a UK-based human cohort. We show that endothelial cells express the putative HCO – 3-sensor receptor-type tyrosine-protein phosphatase RPTPγ, which enhances endothelial intracellular Ca 2+-responses in resistance arteries and facilitates endothelium-dependent vasorelaxation only when CO 2 /HCO – 3 is present. Consistent with waning RPTPγ-dependent vasorelaxation at low [HCO – 3 ], RPTPγ limits increases in cerebral perfusion during neuronal activity and augments decreases in cerebral perfusion during hyperventilation. RPTPγ does not influence resting blood pressure but amplifies hyperventilation-induced blood pressure elevations. Loss-of-function variants in PTPRG, encoding RPTPγ, are associated with increased risk of cerebral infarction, heart attack, and reduced cardiac ejection fraction. We conclude that PTPRG is an ischemia susceptibility locus; and RPTPγ-dependent sensing of HCO – 3 adjusts endothelium-mediated vasorelaxation, microvascular perfusion, and blood pressure during acid-base disturbances and altered tissue metabolism.
Originalsprog | Engelsk |
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Artikelnummer | e57553 |
Tidsskrift | eLife |
Vol/bind | 9 |
Sider (fra-til) | 1-46 |
Antal sider | 46 |
DOI | |
Status | Udgivet - 2020 |