Isolation and expansion of adipose-derived stem cells for tissue engineering

Trine Fink, Jeppe Grøndahl Rasmussen, Pia Lund, Linda Pilgaard, Kjeld Søballe, Vladimir Zachar

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

26 Citationer (Scopus)

Resumé

For treatment of cardiac failure with bone marrow-derived mesenchymal stem cells, several clinical trials are ongoing. However, more attention is gathering on the use of adipose tissue-derived stem cells (ASCs). This paper describes the optimization of isolation and propagation of ASCs for subsequent clinical use. In the isolation step, several enzymes were compared with respect to yield of nucleated cells and precursor cells. Our results showed, that the interdonor variablility was greater than differences between individual enzymes. For propagation of cells, different types of media, sera and serum replacers were evaluated regarding their ability to support cell growth and preserve differentiation potential. Most of serum replacers proved inferior to fetal calf serum. Among the media tested, modified Eagle's media alpha was superior in promoting cell growth while preserving the ability to differentiate. Also, the effect of cell seeding density and hypoxic culture was evaluated. In this study, we show that it is possible to maximize cell yield regardless of donor individual characteristics by simple manipulations of media composition, cell seeding density and gaseous environment.
OriginalsprogEngelsk
TidsskriftFrontiers in Bioscience
Vol/bindE3
Udgave nummer1
Sider (fra-til)256-263
Antal sider8
ISSN1093-9946
DOI
StatusUdgivet - 2011

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Cell Engineering
Tissue Engineering
Stem cells
Tissue engineering
Stem Cells
Cell growth
Tissue
Aptitude
Enzymes
Serum
Adipose Tissue
Bone
Cell Count
Eagles
Growth
Treatment Failure
Chemical analysis
Mesenchymal Stromal Cells
Individuality
Heart Failure

Citer dette

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abstract = "For treatment of cardiac failure with bone marrow-derived mesenchymal stem cells, several clinical trials are ongoing. However, more attention is gathering on the use of adipose tissue-derived stem cells (ASCs). This paper describes the optimization of isolation and propagation of ASCs for subsequent clinical use. In the isolation step, several enzymes were compared with respect to yield of nucleated cells and precursor cells. Our results showed, that the interdonor variablility was greater than differences between individual enzymes. For propagation of cells, different types of media, sera and serum replacers were evaluated regarding their ability to support cell growth and preserve differentiation potential. Most of serum replacers proved inferior to fetal calf serum. Among the media tested, modified Eagle's media alpha was superior in promoting cell growth while preserving the ability to differentiate. Also, the effect of cell seeding density and hypoxic culture was evaluated. In this study, we show that it is possible to maximize cell yield regardless of donor individual characteristics by simple manipulations of media composition, cell seeding density and gaseous environment.",
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Isolation and expansion of adipose-derived stem cells for tissue engineering. / Fink, Trine; Rasmussen, Jeppe Grøndahl; Lund, Pia; Pilgaard, Linda; Søballe, Kjeld; Zachar, Vladimir.

I: Frontiers in Bioscience, Bind E3, Nr. 1, 2011, s. 256-263.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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AU - Fink, Trine

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AU - Lund, Pia

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AU - Søballe, Kjeld

AU - Zachar, Vladimir

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AB - For treatment of cardiac failure with bone marrow-derived mesenchymal stem cells, several clinical trials are ongoing. However, more attention is gathering on the use of adipose tissue-derived stem cells (ASCs). This paper describes the optimization of isolation and propagation of ASCs for subsequent clinical use. In the isolation step, several enzymes were compared with respect to yield of nucleated cells and precursor cells. Our results showed, that the interdonor variablility was greater than differences between individual enzymes. For propagation of cells, different types of media, sera and serum replacers were evaluated regarding their ability to support cell growth and preserve differentiation potential. Most of serum replacers proved inferior to fetal calf serum. Among the media tested, modified Eagle's media alpha was superior in promoting cell growth while preserving the ability to differentiate. Also, the effect of cell seeding density and hypoxic culture was evaluated. In this study, we show that it is possible to maximize cell yield regardless of donor individual characteristics by simple manipulations of media composition, cell seeding density and gaseous environment.

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