Kinetics of insulin binding and kinase activity of the partially purified insulin receptor from human skeletal muscle

J F Bak, A Handberg, H Beck-Nielsen, O Pedersen

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14 Citationer (Scopus)

Abstract

The kinetics of insulin binding and kinase activity of soluble, partially purified insulin receptors from human skeletal muscle are considered. An equilibrium for insulin binding was obtained within 2 h at 37 degrees C. At lower temperatures the equilibrium for insulin binding was less clearly defined. Dissociation of 125I-labelled insulin was incomplete unless an excess amount of unlabelled insulin was added. Insulin-stimulatable autophosphorylation of the 95 kDa subunit was verified by gel electrophoresis. The kinase activity was measured with the synthetic polypeptide poly(Glu-Tyr(4:1] as a phosphoacceptor. The insulin receptor kinase activity correlated significantly (r = 0.92, P less than 0.0001) to the concentration of high-affinity insulin binding sites in the eluate. Autophosphorylation of the insulin receptor was necessary for the activation of the receptor kinase. When activated the receptor kinase activity was stable for at least 60 min at 21 degrees C with a pH optimum of approx. 7.8, similar to the pH optimum for insulin binding. The non-ionic detergent Triton X-100 inhibited the sensitivity of the receptor kinase to insulin. Insulin stimulated the Vmax of the kinase reaction about 3-fold, decreased the Km for ATP from 35 +/- 5 microM (mean +/- S.E.) to 8 +/- 1 microM (P less than 0.02) and induced a positive cooperativity to ATP with an increase in the Hill coefficient from 1.00 +/- 0.02 to 1.37 +/- 0.07 (P less than 0.05). According to the Hill plots, insulin itself showed no cooperativity with respect to receptor binding or kinase activation.

OriginalsprogEngelsk
TidsskriftBBA General Subjects
Vol/bind1052
Udgave nummer2
Sider (fra-til)306-12
Antal sider7
ISSN0304-4165
StatusUdgivet - 1990
Udgivet eksterntJa

Emneord

  • Adenosine Triphosphate
  • Dose-Response Relationship, Drug
  • Humans
  • Insulin
  • Kinetics
  • Muscles
  • Octoxynol
  • Phosphorylation
  • Polyethylene Glycols
  • Protein Conformation
  • Protein Kinases
  • Receptor, Insulin
  • Substrate Specificity

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