TY - JOUR
T1 - Label-free Quantification Reveals Major Proteomic Changes in Pseudomonas putida F1 During the Exponential Growth Phase
AU - Herbst, Florian-Alexander
AU - Danielsen, Heidi Nolsøe
AU - Wimmer, Reinhard
AU - Nielsen, Per Halkjaer
AU - Dueholm, Morten Simonsen
N1 - This article is protected by copyright. All rights reserved.
PY - 2015/9
Y1 - 2015/9
N2 - The physiological adaptation to stationary growth by Pseudomonas putida F1, a model organism for the degradation of aromatic compounds, was investigated by proteome-wide label-free quantification. The data unveiled that entrance to the stationary phase did not involve an abrupt switch within the P. putida F1 proteome, but rather an ongoing adaptation that started already during the mid-exponential growth phase. The proteomic adaptations involved a clear increase in amino acid degradation capabilities and a loss of transcriptional as well as translational capacity. The final entrance to the stationary phase was accompanied by increased oxidative stress protection, although the stress and stationary sigma factor RpoS increased in abundance already during mid-exponential growth. The results show that it is important to consider significant sample variations when exponentially growing cultures are studied alone or compared across proteomic or transcriptomic literature. This article is protected by copyright. All rights reserved.
AB - The physiological adaptation to stationary growth by Pseudomonas putida F1, a model organism for the degradation of aromatic compounds, was investigated by proteome-wide label-free quantification. The data unveiled that entrance to the stationary phase did not involve an abrupt switch within the P. putida F1 proteome, but rather an ongoing adaptation that started already during the mid-exponential growth phase. The proteomic adaptations involved a clear increase in amino acid degradation capabilities and a loss of transcriptional as well as translational capacity. The final entrance to the stationary phase was accompanied by increased oxidative stress protection, although the stress and stationary sigma factor RpoS increased in abundance already during mid-exponential growth. The results show that it is important to consider significant sample variations when exponentially growing cultures are studied alone or compared across proteomic or transcriptomic literature. This article is protected by copyright. All rights reserved.
U2 - 10.1002/pmic.201400482
DO - 10.1002/pmic.201400482
M3 - Journal article
C2 - 26122999
SN - 1615-9853
VL - 15
SP - 3244
EP - 3252
JO - Proteomics
JF - Proteomics
IS - 18
ER -