Maintaining RNA integrity for transcriptomic profiling of ex vivo cultured limbal epithelial stem cells after fluorescence-activated cell sorting (FACS)

Lei Liu, Frederik Mølgaard Nielsen, Simone Elkjær Riis, Jeppe Emmersen, Trine Fink, Jesper Østergaard Hjortdal, Chris Bath, Vladimir Zachar*

*Kontaktforfatter

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

2 Citationer (Scopus)
235 Downloads (Pure)
OriginalsprogEngelsk
Artikelnummer15
TidsskriftBiological Procedures Online
Vol/bind19
Antal sider7
ISSN1480-9222
DOI
StatusUdgivet - 12 dec. 2017

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Stem cells
Sorting
Flow Cytometry
Stem Cells
Epithelial Cells
Fluorescence
Cells
RNA
RNA Sequence Analysis
Ethanol
High-Throughput Nucleotide Sequencing
Fixatives
Intracellular Membranes
Physiology
Genome
Antigens
Messenger RNA
Pipelines
Genes
Membranes

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title = "Maintaining RNA integrity for transcriptomic profiling of ex vivo cultured limbal epithelial stem cells after fluorescence-activated cell sorting (FACS)",
keywords = "Ethanol, Fluorescence-activated cell sorting, Intracellular staining, Limbal stem cells, RNA integrity, RNA sequencing",
author = "Lei Liu and Nielsen, {Frederik M{\o}lgaard} and Riis, {Simone Elkj{\ae}r} and Jeppe Emmersen and Trine Fink and Hjortdal, {Jesper {\O}stergaard} and Chris Bath and Vladimir Zachar",
year = "2017",
month = "12",
day = "12",
doi = "10.1186/s12575-017-0065-2",
language = "English",
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journal = "Biological Procedures Online",
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}

Maintaining RNA integrity for transcriptomic profiling of ex vivo cultured limbal epithelial stem cells after fluorescence-activated cell sorting (FACS). / Liu, Lei; Nielsen, Frederik Mølgaard; Riis, Simone Elkjær; Emmersen, Jeppe; Fink, Trine; Hjortdal, Jesper Østergaard; Bath, Chris; Zachar, Vladimir.

I: Biological Procedures Online, Bind 19, 15, 12.12.2017.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Maintaining RNA integrity for transcriptomic profiling of ex vivo cultured limbal epithelial stem cells after fluorescence-activated cell sorting (FACS)

AU - Liu, Lei

AU - Nielsen, Frederik Mølgaard

AU - Riis, Simone Elkjær

AU - Emmersen, Jeppe

AU - Fink, Trine

AU - Hjortdal, Jesper Østergaard

AU - Bath, Chris

AU - Zachar, Vladimir

PY - 2017/12/12

Y1 - 2017/12/12

KW - Ethanol

KW - Fluorescence-activated cell sorting

KW - Intracellular staining

KW - Limbal stem cells

KW - RNA integrity

KW - RNA sequencing

U2 - 10.1186/s12575-017-0065-2

DO - 10.1186/s12575-017-0065-2

M3 - Journal article

VL - 19

JO - Biological Procedures Online

JF - Biological Procedures Online

SN - 1480-9222

M1 - 15

ER -