Mapping and identification of HeLa cell proteins separated by immobilized pH-gradient two-dimensional gel electrophoresis and construction of a two-dimensional polyacrylamide gel electrophoresis database

Allan Christlan Shaw*, Martin Røssel Larsen, Peter Roepstorff, Arne Holm, Gunna Christiansen, Svend Birkelund

*Kontaktforfatter

Publikation: Bidrag til bog/antologi/rapport/konference proceedingBidrag til bog/antologiForskningpeer review

Abstract

The HeLa cell line, a human adenocarcinoma, is used in many research fields, since it can be infected with a wide range of viruses and intracellular bacteria. Therefore, the mapping of HeLa cell proteins is useful for the investigation of parasite host cell interactions. Because of the recent improvements of two-dimensional gel electrophoresis with immobilized pH gradients (IPG) compared to isoelectric focusing with carrier ampholytes, a highly reproducible method for examining global changes in HeLa cell protein expression due to different stimuli is now available. Therefore, we have initiated the mapping of [35S]methionine/cysteine-labeled HeLa cell proteins with the 2-D PAGE (IPG)-system, using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and Kterminal sequencing for protein identification. To date 21 proteins have been identified and mapped. In order to make these and future data accessible for interlaboratory comparison, we constructed a 2-D PAGE database on the World Wide Web.

OriginalsprogEngelsk
TitelFrom Genome to Proteome: Advances in the Practice & Application of Proteomics
Antal sider7
ForlagWiley-Blackwell
Publikationsdato26 dec. 2007
Sider397-403
ISBN (Trykt)9783527301546
ISBN (Elektronisk)9783527613489
DOI
StatusUdgivet - 26 dec. 2007
Udgivet eksterntJa

Bibliografisk note

Publisher Copyright:
© 2000 WILEY-VCH Verlag GmbH. All rights reserved.

Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.

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