Measuring Host Fitness Effects and Transmission of Wolbachia Strains in Aedes aegypti Mosquitoes

Perran Adam Stott-Ross*

*Kontaktforfatter

Publikation: Bidrag til bog/antologi/rapport/konference proceedingBidrag til bog/antologiForskningpeer review

Abstract

Lines of Aedes aegypti mosquitoes infected with heritable Wolbachia bacteria are being developed and released for arbovirus control. Coordinated releases of lab-reared Wolbachia mosquitoes have reduced local disease incidence by spreading virus-blocking Wolbachia strains and by crashing mosquito populations through incompatible male releases. The phenotypic effects of Wolbachia are diverse and depend on both genetics and the environment. Accurate assessments of Wolbachia effects in mosquitoes are essential, as such effects can make the difference between success and failure of a Wolbachia release program. This chapter provides guidelines for testing key Wolbachia host effects and transmission in Aedes aegypti: the most important arbovirus vector and the most common target of Wolbachia release programs. The protocols should be useful for evaluating mosquito strains prior to field release.

OriginalsprogEngelsk
TitelMethods in Molecular Biology
Antal sider15
Vol/bind2739
ForlagSpringer Publishing Company
Sider189-203
DOI
StatusE-pub ahead of print - 26 nov. 2023
NavnMethods in molecular biology (Clifton, N.J.)
ISSN1064-3745

Bibliografisk note

© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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