Methylphosphonate LNA: a locked nucleic acid with a methylphosphonate linkage

A. Lauritsen; B.M. Dahl; O. Dahl; B. Vester; J. Wengel

doi:10.1016/S0960-894X(02)00882-X

Methylphosphonate LNA: a locked nucleic acid with a methylphosphonate linkage

A. Lauritsen
, B.M. Dahl
, O. Dahl
, B. Vester
, J. Wengel

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

10 Citationer (Scopus)

Abstract

Synthesis of an oligonucleotide containing one methylphosphonate locked nucleic acid (LNA) thymine monomer using the phosphoramidite approach is described. The binding affinity of this 9-mer methylphosphonate LNA towards complementary DNA and RNA oligonucleotides was increased compared to the reference DNA, but decreased compared to the reference LNA. In the 9-mer sequence context studied, introduction of a single methylphosphonate LNA monomer, contrary to a single LNA monomer, efficiently inhibits 3′-exonucleolytic degradation.

Originalsprog	Engelsk
Tidsskrift	Bioorganic & Medicinal Chemistry Letters
Vol/bind	13
Udgave nummer	2
Sider (fra-til)	253-256
ISSN	0960-894X
DOI	https://doi.org/10.1016/S0960-894X(02)00882-X
Status	Udgivet - 2003
Udgivet eksternt	Ja

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10.1016/S0960-894X(02)00882-X

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title = "Methylphosphonate LNA: a locked nucleic acid with a methylphosphonate linkage",

abstract = "Synthesis of an oligonucleotide containing one methylphosphonate locked nucleic acid (LNA) thymine monomer using the phosphoramidite approach is described. The binding affinity of this 9-mer methylphosphonate LNA towards complementary DNA and RNA oligonucleotides was increased compared to the reference DNA, but decreased compared to the reference LNA. In the 9-mer sequence context studied, introduction of a single methylphosphonate LNA monomer, contrary to a single LNA monomer, efficiently inhibits 3′-exonucleolytic degradation. ",

author = "A. Lauritsen and B.M. Dahl and O. Dahl and B. Vester and J. Wengel",

year = "2003",

doi = "10.1016/S0960-894X(02)00882-X",

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TY - JOUR

T1 - Methylphosphonate LNA

T2 - a locked nucleic acid with a methylphosphonate linkage

AU - Lauritsen, A.

AU - Dahl, B.M.

AU - Dahl, O.

AU - Vester, B.

AU - Wengel, J.

PY - 2003

Y1 - 2003

N2 - Synthesis of an oligonucleotide containing one methylphosphonate locked nucleic acid (LNA) thymine monomer using the phosphoramidite approach is described. The binding affinity of this 9-mer methylphosphonate LNA towards complementary DNA and RNA oligonucleotides was increased compared to the reference DNA, but decreased compared to the reference LNA. In the 9-mer sequence context studied, introduction of a single methylphosphonate LNA monomer, contrary to a single LNA monomer, efficiently inhibits 3′-exonucleolytic degradation.

AB - Synthesis of an oligonucleotide containing one methylphosphonate locked nucleic acid (LNA) thymine monomer using the phosphoramidite approach is described. The binding affinity of this 9-mer methylphosphonate LNA towards complementary DNA and RNA oligonucleotides was increased compared to the reference DNA, but decreased compared to the reference LNA. In the 9-mer sequence context studied, introduction of a single methylphosphonate LNA monomer, contrary to a single LNA monomer, efficiently inhibits 3′-exonucleolytic degradation.

U2 - 10.1016/S0960-894X(02)00882-X

DO - 10.1016/S0960-894X(02)00882-X

M3 - Journal article

SN - 0960-894X

VL - 13

SP - 253

EP - 256

JO - Bioorganic & Medicinal Chemistry Letters

JF - Bioorganic & Medicinal Chemistry Letters

IS - 2

ER -

Methylphosphonate LNA: a locked nucleic acid with a methylphosphonate linkage

Abstract

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