TY - JOUR
T1 - Microbiological diagnostics of bloodstream infections in Europe - An ESGBIES survey
AU - Idelevich, Evgeny A
AU - Seifert, Harald
AU - Sundqvist, Martin
AU - Scudeller, Luigia
AU - Amit, Sharon
AU - Balode, Arta
AU - Bilozor, Anastasia
AU - Drevinek, Pavel
AU - Tufan, Zeliha Kocak
AU - Koraqi, Andi
AU - Lamy, Brigitte
AU - Mareković, Ivana
AU - Miciuleviciene, Jolanta
AU - Premru, Manica Müller
AU - Pascual, Alvaro
AU - Pournaras, Spyros
AU - Saegeman, Veroniek
AU - Schønheyder, Henrik Carl
AU - Schrenzel, Jacques
AU - Strateva, Tanya
AU - Tilley, Robert
AU - Wiersinga, W Joost
AU - Zabicka, Dorota
AU - Carmeli, Yehuda
AU - Becker, Karsten
AU - ESCMID Study Group for Bloodstream Infections, Endocarditis and Sepsis (ESGBIES)
N1 - Copyright © 2019 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
PY - 2019/11
Y1 - 2019/11
N2 - Objectives: High-quality diagnosis of bloodstream infections (BSI) is important for successful patient management. As knowledge on current practices of microbiological BSI diagnostics is limited, this project aimed to assess its current state in European microbiological laboratories. Methods: We performed an online questionnaire-based cross-sectional survey comprising 34 questions on practices of microbiological BSI diagnostics. The ESCMID Study Group for Bloodstream Infections, Endocarditis and Sepsis (ESGBIES) was the primary platform to engage national coordinators who recruited laboratories within their countries. Results: Responses were received from 209 laboratories in 25 European countries. Although 32.5% (68/209) of laboratories only used the classical processing of positive blood cultures (BC), two-thirds applied rapid technologies. Of laboratories that provided data, 42.2% (78/185) were able to start incubating BC in automated BC incubators around-the-clock, and only 13% (25/192) had established a 24-h service to start immediate processing of positive BC. Only 4.7% (9/190) of laboratories validated and transmitted the results of identification and antimicrobial susceptibility testing (AST) of BC pathogens to clinicians 24 h/day. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry from briefly incubated sub-cultures on solid media was the most commonly used approach to rapid pathogen identification from positive BC, and direct disc diffusion was the most common rapid AST method from positive BC. Conclusions: Laboratories have started to implement novel technologies for rapid identification and AST for positive BC. However, progress is severely compromised by limited operating hours such that current practice of BC diagnostics in Europe complies only partly with the requirements for optimal BSI management.
AB - Objectives: High-quality diagnosis of bloodstream infections (BSI) is important for successful patient management. As knowledge on current practices of microbiological BSI diagnostics is limited, this project aimed to assess its current state in European microbiological laboratories. Methods: We performed an online questionnaire-based cross-sectional survey comprising 34 questions on practices of microbiological BSI diagnostics. The ESCMID Study Group for Bloodstream Infections, Endocarditis and Sepsis (ESGBIES) was the primary platform to engage national coordinators who recruited laboratories within their countries. Results: Responses were received from 209 laboratories in 25 European countries. Although 32.5% (68/209) of laboratories only used the classical processing of positive blood cultures (BC), two-thirds applied rapid technologies. Of laboratories that provided data, 42.2% (78/185) were able to start incubating BC in automated BC incubators around-the-clock, and only 13% (25/192) had established a 24-h service to start immediate processing of positive BC. Only 4.7% (9/190) of laboratories validated and transmitted the results of identification and antimicrobial susceptibility testing (AST) of BC pathogens to clinicians 24 h/day. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry from briefly incubated sub-cultures on solid media was the most commonly used approach to rapid pathogen identification from positive BC, and direct disc diffusion was the most common rapid AST method from positive BC. Conclusions: Laboratories have started to implement novel technologies for rapid identification and AST for positive BC. However, progress is severely compromised by limited operating hours such that current practice of BC diagnostics in Europe complies only partly with the requirements for optimal BSI management.
KW - Antimicrobial susceptibility testing
KW - Blood culture
KW - Bloodstream infection
KW - Identification
KW - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
KW - Microbiological diagnostics
KW - Molecular methods
KW - Preanalytics
KW - Rapid diagnostics
KW - Transport
UR - http://www.scopus.com/inward/record.url?scp=85064927303&partnerID=8YFLogxK
U2 - 10.1016/j.cmi.2019.03.024
DO - 10.1016/j.cmi.2019.03.024
M3 - Journal article
C2 - 30980927
SN - 1198-743X
VL - 25
SP - 1399
EP - 1407
JO - Clinical Microbiology and Infection
JF - Clinical Microbiology and Infection
IS - 11
ER -