TY - JOUR
T1 - Monitoring of biogas test plants
T2 - A process analytical technology approach
AU - Holm-Nielsen, Jens Bo
AU - Esbensen, Kim H.
PY - 2011/7/15
Y1 - 2011/7/15
N2 - Most studies reported in the literature have investigated near infrared spectroscopy (NIR) in laboratory-scale or minor pilot biogas plants only; practically no other studies have examined the potential for meso-scale/full-scale on-line process monitoring. The focus of this study is on a meso-scale biogas test plant implementation of process analytical technologies (PAT) to develop multivariate calibration/prediction models for anaerobic digestion (AD) processes. A 150 L bioreactor was fitted with a recurrent loop at which NIR spectroscopy and attendant reference sampling were carried out. In all realistic bioreactor scales, it is necessary to obtain a fairly constant level of volatile fatty acid (VFA) concentration, which furthers a stable biogas production. Uncontrolled VFA contents have a significant negative impact on biogas production; VFA concentrations should not exceed 5–6000 mg/L lest the microorganism population may suffer fatal reductions. On-line control and management of VFA concentration levels are therefore critical in order to be able to speed up or slow down the AD processes which produce the desired sustainable bioenergy for combined heat and power production. By calibrating pilot plant NIR spectra to laboratory VFA reference concentrations at the experimental locality Bygholm, it was possible to develop calibration models by partial least squares (PLS) regression, which displayed acceptable to very good prediction performances for total VFA as well as for three other essential individual acids based on test set validations. The average statistics assessing prediction performance, accuracy (slope) and precision (explained variance r2), were both 0.92, which must be considered excellent for this type of significantly heterogeneous systems. The meso- to full-scale feasibility has thus been proven, which has important positive implications for robust, sufficient and reliable, low-cost PAT monitoring systems in the biogas arena and other continuous fermentation processes.
AB - Most studies reported in the literature have investigated near infrared spectroscopy (NIR) in laboratory-scale or minor pilot biogas plants only; practically no other studies have examined the potential for meso-scale/full-scale on-line process monitoring. The focus of this study is on a meso-scale biogas test plant implementation of process analytical technologies (PAT) to develop multivariate calibration/prediction models for anaerobic digestion (AD) processes. A 150 L bioreactor was fitted with a recurrent loop at which NIR spectroscopy and attendant reference sampling were carried out. In all realistic bioreactor scales, it is necessary to obtain a fairly constant level of volatile fatty acid (VFA) concentration, which furthers a stable biogas production. Uncontrolled VFA contents have a significant negative impact on biogas production; VFA concentrations should not exceed 5–6000 mg/L lest the microorganism population may suffer fatal reductions. On-line control and management of VFA concentration levels are therefore critical in order to be able to speed up or slow down the AD processes which produce the desired sustainable bioenergy for combined heat and power production. By calibrating pilot plant NIR spectra to laboratory VFA reference concentrations at the experimental locality Bygholm, it was possible to develop calibration models by partial least squares (PLS) regression, which displayed acceptable to very good prediction performances for total VFA as well as for three other essential individual acids based on test set validations. The average statistics assessing prediction performance, accuracy (slope) and precision (explained variance r2), were both 0.92, which must be considered excellent for this type of significantly heterogeneous systems. The meso- to full-scale feasibility has thus been proven, which has important positive implications for robust, sufficient and reliable, low-cost PAT monitoring systems in the biogas arena and other continuous fermentation processes.
KW - On-line monitoring
KW - Near infrared spectroscopy (NIR)
KW - Anaerobic digestion (AD)
KW - Volatile fatty acids (VFA)
KW - Process analytical technologies (PAT)
KW - Representative sampling
KW - Theory of sampling (TOS)
KW - Biogas production
KW - Biorefinery
KW - Monitoring system for continuous fermentation processes
U2 - 10.1002/cem.1344
DO - 10.1002/cem.1344
M3 - Journal article
SN - 0886-9383
VL - 25
SP - 357
EP - 365
JO - Journal of Chemometrics
JF - Journal of Chemometrics
IS - 7
ER -