Motion analysis of the cervical spine during extension and flexion: Reliability of the vertebral marking procedure

Cervical spine motion analysis using videofluoroscopy is currently a technique without a gold standard. We demonstrate the reliability of a rigid and reliable analysis methodology for cervical motion using videofluoroscopic images, representing the entire range of motion during flexion and extension, from the neutral position to the end-range in the sagittal plane. Two researchers with radiography and vertebral marking expertise, and two inexperienced researchers with 10 hours of training manually marked anatomical structures on fluoroscopic images in a procedure designed to control for vertebral rotation around the mid-plane axis. The average marking error across examiners and images was -0.12∘ (standard deviation: 0.88°), and the intraexaminer error ranged from -1.00∘ to 1.61° (standard deviation range: 0.27°-1.19°). Our method demonstrated lower errors compared to the higher resolution X-ray studies, and proved that vertebral marking can be performed by persons with no experience in radiographic image analysis.