Non-culture-based studies of the appendiceal microbiota: a systematic review

Jacob Antonsen; Matilde Winther-Jensen; Lasse T Krogsbøll; Tine Jess; Lars N Jorgensen; Kristine H Allin

doi:10.2217/fmb-2022-0194

Non-culture-based studies of the appendiceal microbiota: a systematic review

Jacob Antonsen^*, Matilde Winther-Jensen, Lasse T Krogsbøll, Tine Jess, Lars N Jorgensen, Kristine H Allin

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Publikation: Bidrag til tidsskrift › Review (oversigtsartikel) › peer review

2 Citationer (Scopus)

Abstract

Aims: To review studies examining the appendiceal microbiota and microbial changes in acute appendicitis.

Methods: After a systematic literature search, 11 studies examining the appendiceal microbiota (414 samples) using non-culture-based methods were included.

Results: The appendiceal microbiota showed decreased α-diversity compared with fecal microbiota. Inflamed and uninflamed appendices showed differences in β-diversity, and there was an increased abundance of oral-associated bacteria in inflamed versus uninflamed appendices.

Conclusion: The appendiceal microbiota exhibits lower α-diversity than the fecal microbiota, with an increased abundance of oral-associated bacteria. Compared with uninflamed appendices, the appendix microbiota in acute appendicitis also showed increased abundance of oral-associated bacteria, but no bacterial profile unique to either complicated or uncomplicated appendicitis was found.

Originalsprog	Engelsk
Tidsskrift	Future microbiology
Vol/bind	18
Udgave nummer	3
Sider (fra-til)	205-216
Antal sider	12
ISSN	1746-0913
DOI	https://doi.org/10.2217/fmb-2022-0194
Status	Udgivet - 14 mar. 2023

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10.2217/fmb-2022-0194

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Non-culture-based studies of the appendiceal microbiota: a systematic review - Supplementary material FM
Antonsen, J. (Ophavsperson), Winther-Jensen, M. (Ophavsperson), Krogsboll, L. T. (Bidrager), Jess, T. (Ophavsperson), Jorgensen, L. N. (Ophavsperson) & Allin, K. (Ophavsperson), Future Science Group, 2023
DOI: 10.25402/fmb.22269661.v1, https://future-science-group.figshare.com/articles/dataset/Non-culture-based_studies_of_the_appendiceal_microbiota_a_systematic_review_-_Supplementary_material_FM/22269661/1
Datasæt: Supplerende materiale
Non-culture-based studies of the appendiceal microbiota: a systematic review - Table S1
Antonsen, J. (Ophavsperson), Winther-Jensen, M. (Ophavsperson), Krogsboll, L. T. (Bidrager), Jess, T. (Ophavsperson), Jorgensen, L. N. (Ophavsperson) & Allin, K. (Ophavsperson), Future Science Group, 2023
DOI: 10.25402/fmb.22269658.v1, https://future-science-group.figshare.com/articles/dataset/Non-culture-based_studies_of_the_appendiceal_microbiota_a_systematic_review_-_Table_S1/22269658/1
Datasæt
Non-culture-based studies of the appendiceal microbiota: a systematic review - Methods-Reporting-Checklist_FM
Antonsen, J. (Ophavsperson), Winther-Jensen, M. (Ophavsperson), Krogsboll, L. T. (Bidrager), Jess, T. (Ophavsperson), Jorgensen, L. N. (Ophavsperson) & Allin, K. (Ophavsperson), Future Science Group, 2023
DOI: 10.25402/fmb.22269655.v1, https://future-science-group.figshare.com/articles/dataset/Non-culture-based_studies_of_the_appendiceal_microbiota_a_systematic_review_-_Methods-Reporting-Checklist_FM/22269655/1
Datasæt

Citationsformater

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title = "Non-culture-based studies of the appendiceal microbiota: a systematic review",

abstract = "Aims: To review studies examining the appendiceal microbiota and microbial changes in acute appendicitis. Methods: After a systematic literature search, 11 studies examining the appendiceal microbiota (414 samples) using non-culture-based methods were included. Results: The appendiceal microbiota showed decreased α-diversity compared with fecal microbiota. Inflamed and uninflamed appendices showed differences in β-diversity, and there was an increased abundance of oral-associated bacteria in inflamed versus uninflamed appendices. Conclusion: The appendiceal microbiota exhibits lower α-diversity than the fecal microbiota, with an increased abundance of oral-associated bacteria. Compared with uninflamed appendices, the appendix microbiota in acute appendicitis also showed increased abundance of oral-associated bacteria, but no bacterial profile unique to either complicated or uncomplicated appendicitis was found. Plain language summary: This article represents a summary of the current literature examining the bacteria in the human appendix.We aimed to describe the bacterial community in the appendix and look for evidence of bacterial differences between diseased and healthy appendices, as well as evidence of bacteria being the cause of acute appendicitis. We found that the bacteria in the appendix are different from the bacteria in stool. Furthermore, bacteria are different when comparing diseased and healthy appendices. The diseased appendix had more types of bacteria that are normally found in the mouth than the healthy appendix. Our summary did not find any evidence that bacteria are the main cause of developing acute appendicitis. Tweetable abstract: This review found a lower ?-diversity of the appendiceal microbiota compared with the fecal microbiota and an increased presence of bacteria commonly found in the oral cavity in the appendix. Acute appendicitis is characterized by an altered microbiota with increased abundance of Fusobacteria.",

keywords = "Acute Disease, Appendectomy, Appendicitis/microbiology, Appendix/microbiology, Bacteria/genetics, Humans, Microbiota, microbiota, appendix, appendicitis, 16S rRNA gene sequencing",

author = "Jacob Antonsen and Matilde Winther-Jensen and Krogsb{\o}ll, {Lasse T} and Tine Jess and Jorgensen, {Lars N} and Allin, {Kristine H}",

year = "2023",

month = mar,

day = "14",

doi = "10.2217/fmb-2022-0194",

language = "English",

volume = "18",

pages = "205--216",

journal = "Future microbiology",

issn = "1746-0913",

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TY - JOUR

T1 - Non-culture-based studies of the appendiceal microbiota

T2 - a systematic review

AU - Antonsen, Jacob

AU - Winther-Jensen, Matilde

AU - Krogsbøll, Lasse T

AU - Jess, Tine

AU - Jorgensen, Lars N

AU - Allin, Kristine H

PY - 2023/3/14

Y1 - 2023/3/14

N2 - Aims: To review studies examining the appendiceal microbiota and microbial changes in acute appendicitis. Methods: After a systematic literature search, 11 studies examining the appendiceal microbiota (414 samples) using non-culture-based methods were included. Results: The appendiceal microbiota showed decreased α-diversity compared with fecal microbiota. Inflamed and uninflamed appendices showed differences in β-diversity, and there was an increased abundance of oral-associated bacteria in inflamed versus uninflamed appendices. Conclusion: The appendiceal microbiota exhibits lower α-diversity than the fecal microbiota, with an increased abundance of oral-associated bacteria. Compared with uninflamed appendices, the appendix microbiota in acute appendicitis also showed increased abundance of oral-associated bacteria, but no bacterial profile unique to either complicated or uncomplicated appendicitis was found. Plain language summary: This article represents a summary of the current literature examining the bacteria in the human appendix.We aimed to describe the bacterial community in the appendix and look for evidence of bacterial differences between diseased and healthy appendices, as well as evidence of bacteria being the cause of acute appendicitis. We found that the bacteria in the appendix are different from the bacteria in stool. Furthermore, bacteria are different when comparing diseased and healthy appendices. The diseased appendix had more types of bacteria that are normally found in the mouth than the healthy appendix. Our summary did not find any evidence that bacteria are the main cause of developing acute appendicitis. Tweetable abstract: This review found a lower ?-diversity of the appendiceal microbiota compared with the fecal microbiota and an increased presence of bacteria commonly found in the oral cavity in the appendix. Acute appendicitis is characterized by an altered microbiota with increased abundance of Fusobacteria.

AB - Aims: To review studies examining the appendiceal microbiota and microbial changes in acute appendicitis. Methods: After a systematic literature search, 11 studies examining the appendiceal microbiota (414 samples) using non-culture-based methods were included. Results: The appendiceal microbiota showed decreased α-diversity compared with fecal microbiota. Inflamed and uninflamed appendices showed differences in β-diversity, and there was an increased abundance of oral-associated bacteria in inflamed versus uninflamed appendices. Conclusion: The appendiceal microbiota exhibits lower α-diversity than the fecal microbiota, with an increased abundance of oral-associated bacteria. Compared with uninflamed appendices, the appendix microbiota in acute appendicitis also showed increased abundance of oral-associated bacteria, but no bacterial profile unique to either complicated or uncomplicated appendicitis was found. Plain language summary: This article represents a summary of the current literature examining the bacteria in the human appendix.We aimed to describe the bacterial community in the appendix and look for evidence of bacterial differences between diseased and healthy appendices, as well as evidence of bacteria being the cause of acute appendicitis. We found that the bacteria in the appendix are different from the bacteria in stool. Furthermore, bacteria are different when comparing diseased and healthy appendices. The diseased appendix had more types of bacteria that are normally found in the mouth than the healthy appendix. Our summary did not find any evidence that bacteria are the main cause of developing acute appendicitis. Tweetable abstract: This review found a lower ?-diversity of the appendiceal microbiota compared with the fecal microbiota and an increased presence of bacteria commonly found in the oral cavity in the appendix. Acute appendicitis is characterized by an altered microbiota with increased abundance of Fusobacteria.

KW - Acute Disease

KW - Appendectomy

KW - Appendicitis/microbiology

KW - Appendix/microbiology

KW - Bacteria/genetics

KW - Humans

KW - Microbiota

KW - microbiota

KW - appendix

KW - appendicitis

KW - 16S rRNA gene sequencing

UR - http://www.scopus.com/inward/record.url?scp=85152155229&partnerID=8YFLogxK

U2 - 10.2217/fmb-2022-0194

DO - 10.2217/fmb-2022-0194

M3 - Review article

C2 - 36916537

SN - 1746-0913

VL - 18

SP - 205

EP - 216

JO - Future microbiology

JF - Future microbiology

IS - 3

ER -

Non-culture-based studies of the appendiceal microbiota: a systematic review

Abstract

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Non-culture-based studies of the appendiceal microbiota: a systematic review - Supplementary material FM

Non-culture-based studies of the appendiceal microbiota: a systematic review - Table S1

Non-culture-based studies of the appendiceal microbiota: a systematic review - Methods-Reporting-Checklist_FM

Citationsformater