Novel fungal proteins in the chalkbrood infection of honey bee larvae

Doris Roth, Annette Bruun Jensen, Morten Nedergaard Grell, Lene Lange

Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskning

Resumé

Discovery of novel fungal secreted proteins not only shed light on the biology of the secreting organism it may also lead to industrial exploitation. We expect that this is especially true for investigating the interaction between two organisms, which largely relies on secreted protein signals. Here we investigate the interaction between the honey bee and its fungal pathogen Ascosphaera apis, the causative agent of chalkbrood, by identifying enzymes secreted by bee and fungus during different timepoints of infection. Upon testing A. apis-infected larvae for enzyme activity, the larvae exhibiting significant activity were used to produce cDNA libraries. These dual organism cDNA libraries were then screened by transposon-assisted signal sequence trapping (TAST), a method well established for identifying genes for secreted proteins (Becker et al. (2004) J Microbiol Methods 57:123-133). After the trappants are sequenced and annotated, selected genes are further described. As a result, we will deepen the understanding of chalkbrood, one of the main honey bee pests with relevant impact on the economy, among others due to the essential role of bees in pollination.
OriginalsprogEngelsk
TidsskriftFungal Genetics Reports
Vol/bind56 (Suppl)
Sider (fra-til)456
ISSN1941-4757
StatusUdgivet - 2009
BegivenhedThe 25th Fungal Genetics Conference at Asilomar - Pacific Grove, USA
Varighed: 17 mar. 200922 mar. 2009

Konference

KonferenceThe 25th Fungal Genetics Conference at Asilomar
LandUSA
ByPacific Grove
Periode17/03/200922/03/2009

Fingerprint

chalk brood
fungal proteins
Ascosphaera apis
insect larvae
cDNA libraries
honey bees
Apoidea
organisms
infection
proteins
larvae
signal peptide
transposons
trapping
pollination
genes
pests
enzyme activity
Biological Sciences
fungi

Citer dette

Roth, D., Jensen, A. B., Grell, M. N., & Lange, L. (2009). Novel fungal proteins in the chalkbrood infection of honey bee larvae. Fungal Genetics Reports, 56 (Suppl), 456.
Roth, Doris ; Jensen, Annette Bruun ; Grell, Morten Nedergaard ; Lange, Lene. / Novel fungal proteins in the chalkbrood infection of honey bee larvae. I: Fungal Genetics Reports. 2009 ; Bind 56 (Suppl). s. 456.
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Roth, D, Jensen, AB, Grell, MN & Lange, L 2009, 'Novel fungal proteins in the chalkbrood infection of honey bee larvae', Fungal Genetics Reports, bind 56 (Suppl), s. 456.

Novel fungal proteins in the chalkbrood infection of honey bee larvae. / Roth, Doris; Jensen, Annette Bruun; Grell, Morten Nedergaard; Lange, Lene.

I: Fungal Genetics Reports, Bind 56 (Suppl), 2009, s. 456.

Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskning

TY - ABST

T1 - Novel fungal proteins in the chalkbrood infection of honey bee larvae

AU - Roth, Doris

AU - Jensen, Annette Bruun

AU - Grell, Morten Nedergaard

AU - Lange, Lene

PY - 2009

Y1 - 2009

N2 - Discovery of novel fungal secreted proteins not only shed light on the biology of the secreting organism it may also lead to industrial exploitation. We expect that this is especially true for investigating the interaction between two organisms, which largely relies on secreted protein signals. Here we investigate the interaction between the honey bee and its fungal pathogen Ascosphaera apis, the causative agent of chalkbrood, by identifying enzymes secreted by bee and fungus during different timepoints of infection. Upon testing A. apis-infected larvae for enzyme activity, the larvae exhibiting significant activity were used to produce cDNA libraries. These dual organism cDNA libraries were then screened by transposon-assisted signal sequence trapping (TAST), a method well established for identifying genes for secreted proteins (Becker et al. (2004) J Microbiol Methods 57:123-133). After the trappants are sequenced and annotated, selected genes are further described. As a result, we will deepen the understanding of chalkbrood, one of the main honey bee pests with relevant impact on the economy, among others due to the essential role of bees in pollination.

AB - Discovery of novel fungal secreted proteins not only shed light on the biology of the secreting organism it may also lead to industrial exploitation. We expect that this is especially true for investigating the interaction between two organisms, which largely relies on secreted protein signals. Here we investigate the interaction between the honey bee and its fungal pathogen Ascosphaera apis, the causative agent of chalkbrood, by identifying enzymes secreted by bee and fungus during different timepoints of infection. Upon testing A. apis-infected larvae for enzyme activity, the larvae exhibiting significant activity were used to produce cDNA libraries. These dual organism cDNA libraries were then screened by transposon-assisted signal sequence trapping (TAST), a method well established for identifying genes for secreted proteins (Becker et al. (2004) J Microbiol Methods 57:123-133). After the trappants are sequenced and annotated, selected genes are further described. As a result, we will deepen the understanding of chalkbrood, one of the main honey bee pests with relevant impact on the economy, among others due to the essential role of bees in pollination.

KW - Parasitic interactions

KW - Fungus-host interactions

KW - Entomopathogens

KW - Gene discovery

KW - Secretome

KW - Extracellular proteins

M3 - Conference abstract in journal

VL - 56 (Suppl)

SP - 456

JO - Fungal Genetics Reports

JF - Fungal Genetics Reports

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ER -

Roth D, Jensen AB, Grell MN, Lange L. Novel fungal proteins in the chalkbrood infection of honey bee larvae. Fungal Genetics Reports. 2009;56 (Suppl):456.