Novel insights into the use of glowing LNA as nucleic acid detection probes-Influence of labeling density and nucleobases.

Michael E. Østergaard; Jyotirmoy Maity; B. Ravindra Babu; Jesper Wengel; Patrick J Hrdlicka

doi:10.1016/j.bmcl.2010.10.089

Novel insights into the use of glowing LNA as nucleic acid detection probes-Influence of labeling density and nucleobases.

Michael E. Østergaard
, Jyotirmoy Maity
, B. Ravindra Babu
, Jesper Wengel
, Patrick J Hrdlicka

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

9 Citationer (Scopus)

Abstract

Appropriately designed 2′-N-(pyren-1-yl)carbonyl-2′-amino-LNA (locked nucleic acid) display large increases in fluorescence intensity and remarkably high quantum yields upon hybridization with nucleic acid targets. Thermal denaturation and fluorescence spectroscopy studies on ONs modified with known thymine monomer X and novel 5-methylcytosine monomer Y provide new insights into the design principles and mechanism of these Glowing LNA nucleic acid detection probes.

Originalsprog	Engelsk
Tidsskrift	Bioorganic & Medicinal Chemistry Letters
Vol/bind	20
Udgave nummer	24
Sider (fra-til)	7265-7268
ISSN	0960-894X
DOI	https://doi.org/10.1016/j.bmcl.2010.10.089
Status	Udgivet - 2010
Udgivet eksternt	Ja

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10.1016/j.bmcl.2010.10.089

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title = "Novel insights into the use of glowing LNA as nucleic acid detection probes-Influence of labeling density and nucleobases.",

abstract = "Appropriately designed 2′-N-(pyren-1-yl)carbonyl-2′-amino-LNA (locked nucleic acid) display large increases in fluorescence intensity and remarkably high quantum yields upon hybridization with nucleic acid targets. Thermal denaturation and fluorescence spectroscopy studies on ONs modified with known thymine monomer X and novel 5-methylcytosine monomer Y provide new insights into the design principles and mechanism of these Glowing LNA nucleic acid detection probes.",

author = "{\O}stergaard, \{Michael E.\} and Jyotirmoy Maity and Babu, \{B. Ravindra\} and Jesper Wengel and Hrdlicka, \{Patrick J\}",

year = "2010",

doi = "10.1016/j.bmcl.2010.10.089",

language = "English",

volume = "20",

pages = "7265--7268",

journal = "Bioorganic \& Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier",

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TY - JOUR

T1 - Novel insights into the use of glowing LNA as nucleic acid detection probes-Influence of labeling density and nucleobases.

AU - Østergaard, Michael E.

AU - Maity, Jyotirmoy

AU - Babu, B. Ravindra

AU - Wengel, Jesper

AU - Hrdlicka, Patrick J

PY - 2010

Y1 - 2010

N2 - Appropriately designed 2′-N-(pyren-1-yl)carbonyl-2′-amino-LNA (locked nucleic acid) display large increases in fluorescence intensity and remarkably high quantum yields upon hybridization with nucleic acid targets. Thermal denaturation and fluorescence spectroscopy studies on ONs modified with known thymine monomer X and novel 5-methylcytosine monomer Y provide new insights into the design principles and mechanism of these Glowing LNA nucleic acid detection probes.

AB - Appropriately designed 2′-N-(pyren-1-yl)carbonyl-2′-amino-LNA (locked nucleic acid) display large increases in fluorescence intensity and remarkably high quantum yields upon hybridization with nucleic acid targets. Thermal denaturation and fluorescence spectroscopy studies on ONs modified with known thymine monomer X and novel 5-methylcytosine monomer Y provide new insights into the design principles and mechanism of these Glowing LNA nucleic acid detection probes.

U2 - 10.1016/j.bmcl.2010.10.089

DO - 10.1016/j.bmcl.2010.10.089

M3 - Journal article

C2 - 21071224

SN - 0960-894X

VL - 20

SP - 7265

EP - 7268

JO - Bioorganic & Medicinal Chemistry Letters

JF - Bioorganic & Medicinal Chemistry Letters

IS - 24

ER -

Novel insights into the use of glowing LNA as nucleic acid detection probes-Influence of labeling density and nucleobases.

Abstract

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