One-step electrochemical approach of enzyme immobilization for bioelectrochemical applications

Fei Shen, Simin Arshi, Edmond Magner, Jens Ulstrup, Xinxin Xiao

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

4 Citationer (Scopus)
18 Downloads (Pure)


Enzymatic bioelectrochemistry represents the marriage of electrochemistry and enzymatic biocatalysis, and has led to important applications for biosensors, biofuel cells, and bioelectrocatalysis. Enzyme immobilization is the basis of enzymatic bioelectrochemistry, as immobilization itself determines the enzyme/material interface and thus the electrochemical performance. Amongst the range of methods of enzyme immobilization, one-step electrochemical approaches feature rapid immobilization and good control over the processes, enabling partial or total use of the electrode surface. In this mini-review, we first briefly introduce the operating principles of bioelectrochemical applications based on enzyme modified electrodes. We then overview recent progress in utilizing conductive polymers, redox-active modified polymers, sol–gel silica and electrochemically assistant adsorption for enzyme immobilization via one-step electrochemical approaches. The use of conductive polymers for in situ enzyme immobilization is our major focus. Perspectives for future work are also described.
TidsskriftSynthetic Metals
StatusUdgivet - 27 okt. 2022


  • Bioelectrochemistry
  • Conductive polymers
  • Electropolymerization
  • Enzyme immobilization
  • One-step approaches


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