Optimizing monosaccharide production from liquid hot water pretreatment and enzymatic hydrolysis of grass-clover press cake

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Abstract

In the present study, clover-grass press cake was treated by liquid hot water at temperatures of 180–200 °C for a reaction time of 5–10 min. Evaluation of pretreatments was based on the monosaccharide yield after enzymatic hydrolysis of the pretreated slurry and solid fraction, respectively. Extraction of up to 48% hemicellulose and 4% cellulose was observed during pretreatment. The optimal pretreatment conditions were identified as 190 °C and 10 min resulting in monosaccharide yields of 90% and 73% of the theoretical maximum by slurry and solid conversion, respectively. At optimal conditions, the C6 monosaccharide yield (83–90%) was fairly equal compared to the C5 monosaccharide yield (56–89%), which increased by slurry conversion due to near-complete monomerization of soluble xylo-oligosaccharides. In this study, we showed that clover-grass press cake possesses considerable potential as feedstock for production of fermentable sugars in a biorefinery context.

OriginalsprogEngelsk
Artikelnummere18448
TidsskriftHeliyon
Vol/bind9
Udgave nummer8
ISSN2405-8440
DOI
StatusUdgivet - aug. 2023

Bibliografisk note

Funding Information:
The companies BioRefine A/S and NIRAS are acknowledged for their cooperation and supply of raw materials. We also want to thank Helle Blendstrup and Anne Vibeke Kofoed Rasmussen for their valuable help in the laboratory.

Publisher Copyright:
© 2023

© 2023 Published by Elsevier Ltd.

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