Oral and Fecal Campylobacter concisus Strains induce Barrier dysfunction by Apoptosis in HT-29/B6 Intestinal Epithelial Cells

Hans Linde Nielsen, Henrik Ib Nielsen, Tove Ejlertsen, Jørgen Engberg, Dorothee Günzel, Martin Zeitz, Nina A Hering, Michael Fromm, Jörg-Dieter Schulzke, Roland Bücker

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskningpeer review

Abstract

Campylobacter concisus infections of the gastrointestinal tract can be accompanied by diarrhea and inflammation, whereas colonization of the human oral cavity might have a commensal nature. We focus on the pathophysiology of C. concisus and the effects of different clinical oral and fecal C. concisus strains on human HT-29/B6 colon cells. Six oral and eight fecal strains of C. concisus were isolated. Mucus-producing HT-29/B6 epithelial monolayers were infected with the C. concisus strains. Transepithelial electrical resistance (R(t)) and tracer fluxes of different molecule size were measured in Ussing chambers. Tight junction (TJ) protein expression was determined by Western blotting, and subcellular TJ distribution was analyzed by confocal laser-scanning microscopy. Apoptosis induction was examined by TUNEL-staining and Western blot of caspase-3 activation. All strains invaded confluent HT-29/B6 cells and impaired epithelial barrier function, characterized by a time- and dose-dependent decrease in R(t) either after infection from the apical side but even more from the basolateral compartment. TJ protein expression changes were sparse, only in apoptotic areas of infected monolayers TJ proteins were redistributed. Solely the barrier-forming TJ protein claudin-5 showed a reduced expression level to 66±8% (P
OriginalsprogEngelsk
Publikationsdato2011
StatusUdgivet - 2011
Udgivet eksterntJa
Begivenhed16th International Workshop on Campylobacter, Helicobacter and Related Organisms - Vancouver
Varighed: 28 aug. 20111 sep. 2011

Konference

Konference16th International Workshop on Campylobacter, Helicobacter and Related Organisms
ByVancouver
Periode28/08/201101/09/2011

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