Abstract
A technique for creating arrays of parallel, stretched single molecules of deoxyribose nucleic acid (DNA) on an arbitrary substrate for high-resolution scanning-probe imaging is discussed. The technique consists of lithographically patterning polystyrene lines on a substrate which then provide attachment sites for the ends of individual DNA molecules. Molecular combing is performed to stretch DNA from one polystyrene line to the other. Scanning-tunneling and atomic-force microscope images of single molecules of bacteriophage-lambda DNA are shown to demonstrate the advantages of this technique. Several applications, from high-resolution genomics to molecular electronics, are discussed.
Originalsprog | Engelsk |
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Tidsskrift | Applied Physics Letters |
Vol/bind | 78 |
Udgave nummer | 16 |
Sider (fra-til) | 2396-2398 |
Antal sider | 3 |
ISSN | 0003-6951 |
DOI | |
Status | Udgivet - 16 apr. 2001 |