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Abstract
Few‐atom silver nanoclusters (AgNCs) can exhibit strong fluorescence; however, they require ligands to prevent aggregation into larger nanoparticles. Fluorescent AgNCs in biopolymer scaffolds have so far mainly been synthesized in solution, and peptides have only found limited use compared to DNA. Herein, we demonstrate how solid‐phase methods can increase throughput dramatically in peptide ligand screening and in initial evaluation of fluorescence intensity and chemical stability of peptide‐stabilized AgNCs (P‐AgNCs). 9‐Fluorenylmethyloxycarbonyl (Fmoc) solid‐phase peptide synthesis on a hydroxymethyl‐benzoic acid (HMBA) polyethylene glycol polyacrylamide copolymer (PEGA) resin enabled on‐resin screening and evaluation of a peptide library, leading to identification of novel peptide‐stabilized, fluorescent AgNCs. Using systematic amino acid substitutions, we synthesized and screened a 144‐member library. This allowed us to evaluate the effect of length, charge, and Cys content in peptides used as ligands for AgNC stabilization. The results of this study will enable future spectroscopic studies of these peptide‐stabilized AgNCs for bioimaging and other applications.
Originalsprog | Engelsk |
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Artikelnummer | 8 |
Tidsskrift | Chemistry: A European Journal |
Vol/bind | 22 |
Udgave nummer | 51 |
Sider (fra-til) | 18492 |
Antal sider | 18.500 |
ISSN | 0947-6539 |
DOI | |
Status | Udgivet - 3 nov. 2016 |
Udgivet eksternt | Ja |
Fingeraftryk
Dyk ned i forskningsemnerne om 'Peptide‐Stabilized, Fluorescent Silver Nanoclusters: Solid‐Phase Synthesis and Screening'. Sammen danner de et unikt fingeraftryk.Aktiviteter
- 1 Konferenceoplæg
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23rd American Peptide Symposium
Simon Gregersen (Oplægsholder)
22 jun. 2013 → 27 jun. 2013Aktivitet: Foredrag og mundtlige bidrag › Konferenceoplæg
Priser
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Flourescent peptide-stabilized silver-nanoclusters, a solid-phase approach for high-throughput ligand discovery
Gregersen, S., 4 feb. 2014, 1 udg. Copenhagen: Copenhagen University. 172 s.Publikation: Ph.d.-afhandling
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Novel Peptide Ligands for Stabilization of Fluorescent, Silver Nanoclusters: On-resin Screening of a Peptide Library
Gregersen, S., Jensen, K. J. & Vosch, T. A. J., 13 maj 2013.Publikation: Konferencebidrag uden forlag/tidsskrift › Poster › Forskning › peer review
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Peptides as Ligands for Fluorescent, Silver Nanoclusters: Development of a Novel Platform for On-resin Screening
Gregersen, S., Jensen, K. J. & Vosch, T. A. J., 13 maj 2013, I: Journal of Peptide Science. 100, s. 294 1 s., YI-P 216.Publikation: Bidrag til tidsskrift › Konferenceabstrakt i tidsskrift › Forskning › peer review
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