Photonic immobilization techniques used for the detection of cardiovascular disease biomarkers

Odete Goncalves, Maria-Jose Banuls, Rafael Alonso, Pilar Jimenez-Meneses, Angel Maquieira, Henrik Vorum, Steffen B. Petersen, Maria Teresa Neves-Petersen

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Resumé

In today's point-of-care testing (POCT), there is an ever-increasing demand for novel and more efficient devices for early diagnosis, especially in cardiovascular diseases (CVD). Early detection of CVD markers, such as Troponin present in the bloodstream, is a key factor for reducing CVD mortality rates. Thiol-ene coupling (TEC) and Light Assisted Molecular Immobilization (LAMI) are photonic techniques leading to immobilization of bioreceptors, such as, antibodies which recognize cardiac markers. These techniques present advantages compared to traditional immobilization techniques since, e.g., there are no thermal or chemical steps and they work in water media. TEC reaction takes place at close-to-visible wavelengths (lambda=365nm) which induces the formation of thiol radicals which bind to alkene functional group on the surface through a thioether bond. LAMI secures molecular immobilizations in a spatially oriented, localized and covalent coupling of biomolecules onto thiol reactive surfaces down to submicrometer spatial resolution. LAMI is possible due to a conserved structural motif in proteins: the spatial proximity between aromatic residues and disulfide bridges. When aromatic residues are excited with UV light (275-295nm), disulphide bridges are disrupted and free thiol groups are formed that can bind covalently to a surface decorated with thiol groups. We have achieved successful immobilization of anti-troponin and anti-myoglobin antibodies with both photonic immobilization techniques. The microarrays of immobilized monoclonal antibodies have successfully detected the CVD biomarkers troponin I and myoglobin, as confirmed by fluorescence imaging. A sandwich immunoassay was carried out, Troponin I and Myoglobin were detected down to 10 ng/mL and 1 ng/mL, respectively.
OriginalsprogUdefineret/Ukendt
TitelBiophotonics : Photonic Solutions for Better Health Care VI
RedaktørerJ Popp, VV Tuchin, FS Pavone
Antal sider15
Vol/bind10685
ForlagSPIE - International Society for Optical Engineering
Publikationsdato2018
Artikelnummer106852Z-1
ISBN (Trykt)978-1-5106-1897-8
DOI
StatusUdgivet - 2018
BegivenhedConference on Biophotonics: Photonic Solutions for Better Health Care - Strasbourg, Frankrig
Varighed: 23 apr. 201826 apr. 2018
Konferencens nummer: VI

Konference

KonferenceConference on Biophotonics
NummerVI
LandFrankrig
ByStrasbourg
Periode23/04/201826/04/2018
NavnProceedings of the Society of Photo-optical Instrumentation Engineers (SPIE)
Vol/bind10685
ISSN0277-786X

Bibliografisk note

Conference on Biophotonics - Photonic Solutions for Better Health Care VI, Strasbourg, FRANCE, APR 23-26, 2018

Citer dette

Goncalves, O., Banuls, M-J., Alonso, R., Jimenez-Meneses, P., Maquieira, A., Vorum, H., ... Neves-Petersen, M. T. (2018). Photonic immobilization techniques used for the detection of cardiovascular disease biomarkers. I J. Popp, VV. Tuchin, & FS. Pavone (red.), Biophotonics: Photonic Solutions for Better Health Care VI (Bind 10685). [106852Z-1] SPIE - International Society for Optical Engineering. Proceedings of the Society of Photo-optical Instrumentation Engineers (SPIE), Bind. 10685 https://doi.org/10.1117/12.2305717
Goncalves, Odete ; Banuls, Maria-Jose ; Alonso, Rafael ; Jimenez-Meneses, Pilar ; Maquieira, Angel ; Vorum, Henrik ; Petersen, Steffen B. ; Neves-Petersen, Maria Teresa. / Photonic immobilization techniques used for the detection of cardiovascular disease biomarkers. Biophotonics: Photonic Solutions for Better Health Care VI. red. / J Popp ; VV Tuchin ; FS Pavone. Bind 10685 SPIE - International Society for Optical Engineering, 2018. (Proceedings of the Society of Photo-optical Instrumentation Engineers (SPIE), Bind 10685).
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title = "Photonic immobilization techniques used for the detection of cardiovascular disease biomarkers",
abstract = "In today's point-of-care testing (POCT), there is an ever-increasing demand for novel and more efficient devices for early diagnosis, especially in cardiovascular diseases (CVD). Early detection of CVD markers, such as Troponin present in the bloodstream, is a key factor for reducing CVD mortality rates. Thiol-ene coupling (TEC) and Light Assisted Molecular Immobilization (LAMI) are photonic techniques leading to immobilization of bioreceptors, such as, antibodies which recognize cardiac markers. These techniques present advantages compared to traditional immobilization techniques since, e.g., there are no thermal or chemical steps and they work in water media. TEC reaction takes place at close-to-visible wavelengths (lambda=365nm) which induces the formation of thiol radicals which bind to alkene functional group on the surface through a thioether bond. LAMI secures molecular immobilizations in a spatially oriented, localized and covalent coupling of biomolecules onto thiol reactive surfaces down to submicrometer spatial resolution. LAMI is possible due to a conserved structural motif in proteins: the spatial proximity between aromatic residues and disulfide bridges. When aromatic residues are excited with UV light (275-295nm), disulphide bridges are disrupted and free thiol groups are formed that can bind covalently to a surface decorated with thiol groups. We have achieved successful immobilization of anti-troponin and anti-myoglobin antibodies with both photonic immobilization techniques. The microarrays of immobilized monoclonal antibodies have successfully detected the CVD biomarkers troponin I and myoglobin, as confirmed by fluorescence imaging. A sandwich immunoassay was carried out, Troponin I and Myoglobin were detected down to 10 ng/mL and 1 ng/mL, respectively.",
author = "Odete Goncalves and Maria-Jose Banuls and Rafael Alonso and Pilar Jimenez-Meneses and Angel Maquieira and Henrik Vorum and Petersen, {Steffen B.} and Neves-Petersen, {Maria Teresa}",
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Goncalves, O, Banuls, M-J, Alonso, R, Jimenez-Meneses, P, Maquieira, A, Vorum, H, Petersen, SB & Neves-Petersen, MT 2018, Photonic immobilization techniques used for the detection of cardiovascular disease biomarkers. i J Popp, VV Tuchin & FS Pavone (red), Biophotonics: Photonic Solutions for Better Health Care VI. bind 10685, 106852Z-1, SPIE - International Society for Optical Engineering, Proceedings of the Society of Photo-optical Instrumentation Engineers (SPIE), bind 10685, Conference on Biophotonics, Strasbourg, Frankrig, 23/04/2018. https://doi.org/10.1117/12.2305717

Photonic immobilization techniques used for the detection of cardiovascular disease biomarkers. / Goncalves, Odete; Banuls, Maria-Jose; Alonso, Rafael; Jimenez-Meneses, Pilar; Maquieira, Angel; Vorum, Henrik; Petersen, Steffen B.; Neves-Petersen, Maria Teresa.

Biophotonics: Photonic Solutions for Better Health Care VI. red. / J Popp; VV Tuchin; FS Pavone. Bind 10685 SPIE - International Society for Optical Engineering, 2018. 106852Z-1 (Proceedings of the Society of Photo-optical Instrumentation Engineers (SPIE), Bind 10685).

Publikation: Bidrag til bog/antologi/rapport/konference proceedingKonferenceartikel i proceedingForskningpeer review

TY - GEN

T1 - Photonic immobilization techniques used for the detection of cardiovascular disease biomarkers

AU - Goncalves, Odete

AU - Banuls, Maria-Jose

AU - Alonso, Rafael

AU - Jimenez-Meneses, Pilar

AU - Maquieira, Angel

AU - Vorum, Henrik

AU - Petersen, Steffen B.

AU - Neves-Petersen, Maria Teresa

N1 - Conference on Biophotonics - Photonic Solutions for Better Health Care VI, Strasbourg, FRANCE, APR 23-26, 2018

PY - 2018

Y1 - 2018

N2 - In today's point-of-care testing (POCT), there is an ever-increasing demand for novel and more efficient devices for early diagnosis, especially in cardiovascular diseases (CVD). Early detection of CVD markers, such as Troponin present in the bloodstream, is a key factor for reducing CVD mortality rates. Thiol-ene coupling (TEC) and Light Assisted Molecular Immobilization (LAMI) are photonic techniques leading to immobilization of bioreceptors, such as, antibodies which recognize cardiac markers. These techniques present advantages compared to traditional immobilization techniques since, e.g., there are no thermal or chemical steps and they work in water media. TEC reaction takes place at close-to-visible wavelengths (lambda=365nm) which induces the formation of thiol radicals which bind to alkene functional group on the surface through a thioether bond. LAMI secures molecular immobilizations in a spatially oriented, localized and covalent coupling of biomolecules onto thiol reactive surfaces down to submicrometer spatial resolution. LAMI is possible due to a conserved structural motif in proteins: the spatial proximity between aromatic residues and disulfide bridges. When aromatic residues are excited with UV light (275-295nm), disulphide bridges are disrupted and free thiol groups are formed that can bind covalently to a surface decorated with thiol groups. We have achieved successful immobilization of anti-troponin and anti-myoglobin antibodies with both photonic immobilization techniques. The microarrays of immobilized monoclonal antibodies have successfully detected the CVD biomarkers troponin I and myoglobin, as confirmed by fluorescence imaging. A sandwich immunoassay was carried out, Troponin I and Myoglobin were detected down to 10 ng/mL and 1 ng/mL, respectively.

AB - In today's point-of-care testing (POCT), there is an ever-increasing demand for novel and more efficient devices for early diagnosis, especially in cardiovascular diseases (CVD). Early detection of CVD markers, such as Troponin present in the bloodstream, is a key factor for reducing CVD mortality rates. Thiol-ene coupling (TEC) and Light Assisted Molecular Immobilization (LAMI) are photonic techniques leading to immobilization of bioreceptors, such as, antibodies which recognize cardiac markers. These techniques present advantages compared to traditional immobilization techniques since, e.g., there are no thermal or chemical steps and they work in water media. TEC reaction takes place at close-to-visible wavelengths (lambda=365nm) which induces the formation of thiol radicals which bind to alkene functional group on the surface through a thioether bond. LAMI secures molecular immobilizations in a spatially oriented, localized and covalent coupling of biomolecules onto thiol reactive surfaces down to submicrometer spatial resolution. LAMI is possible due to a conserved structural motif in proteins: the spatial proximity between aromatic residues and disulfide bridges. When aromatic residues are excited with UV light (275-295nm), disulphide bridges are disrupted and free thiol groups are formed that can bind covalently to a surface decorated with thiol groups. We have achieved successful immobilization of anti-troponin and anti-myoglobin antibodies with both photonic immobilization techniques. The microarrays of immobilized monoclonal antibodies have successfully detected the CVD biomarkers troponin I and myoglobin, as confirmed by fluorescence imaging. A sandwich immunoassay was carried out, Troponin I and Myoglobin were detected down to 10 ng/mL and 1 ng/mL, respectively.

U2 - 10.1117/12.2305717

DO - 10.1117/12.2305717

M3 - Konferenceartikel i proceeding

SN - 978-1-5106-1897-8

VL - 10685

T3 - Proceedings of the Society of Photo-optical Instrumentation Engineers (SPIE)

BT - Biophotonics

A2 - Popp, J

A2 - Tuchin, VV

A2 - Pavone, FS

PB - SPIE - International Society for Optical Engineering

ER -

Goncalves O, Banuls M-J, Alonso R, Jimenez-Meneses P, Maquieira A, Vorum H et al. Photonic immobilization techniques used for the detection of cardiovascular disease biomarkers. I Popp J, Tuchin VV, Pavone FS, red., Biophotonics: Photonic Solutions for Better Health Care VI. Bind 10685. SPIE - International Society for Optical Engineering. 2018. 106852Z-1. (Proceedings of the Society of Photo-optical Instrumentation Engineers (SPIE), Bind 10685). https://doi.org/10.1117/12.2305717