Polyketide synthase from Fusarium: Cloning, expression and purification for structural analysis

Kasper Kvesel, Reinhard Wimmer, Jens Laurids Sørensen, Frederik Hansen, Michael Toft Overgaard, Henriette Giese, Teis Søndergaard

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

Abstract

Fungi produce a wide array of secondary metabolites, with interesting bioactivities by help of a number of enzyme complexes. Polyketide synthases (PKS) are
a class of multidomain enzymes, producing a class of secondary metabolites called polyketides1,2. Only few structures of PKS’s have been described, even
fewer from fungi and none from Fusarium species. Multidomain proteins can be quite challenging to work with, which is why the project intends to solve the
3D-structures of single domains of PKS’s. In this project, the plan is to clone, express and purify the Acyl-carrier protein (ACP) domain from PKS6 in Fusarium
graminearum for structural analysis.
OriginalsprogEngelsk
Publikationsdato23 mar. 2014
StatusUdgivet - 23 mar. 2014
Begivenhed12 European Conference on Fungal Genetics - Seville, Spanien
Varighed: 23 mar. 201428 mar. 2014

Konference

Konference12 European Conference on Fungal Genetics
Land/OmrådeSpanien
BySeville
Periode23/03/201428/03/2014

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