Production of xylanolytic enzymes by Moesziomyces spp. using xylose, xylan and brewery's spent grain as substrates

Nuno Torres Faria, Susana Marques, Frederico Castelo Ferreira*, César Fonseca

*Kontaktforfatter

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

2 Citationer (Scopus)

Resumé

Xylanases play a crucial role in the hydrolysis of xylan-rich hemicelluloses and have wide industrial applications in the fuel, food, feed and pulp and paper industries. The production of these enzymes at low cost is of paramount importance for their commercial deployment. Moesziomyces antarcticus PYCC 5048 T and M. aphidis PYCC 5535 T were screened for their ability to produce xylanolytic enzymes when grown on D-xylose, xylan (beechwood) and brewery's spent grain (BSG). The extracellular crude extracts produced were characterized and tested in xylan hydrolysis. The yeasts produced xylanolytic enzymes without cellulolytic activity on all the substrates tested. The highest xylanase volumetric activity was obtained with M. aphidis PYCC 5535 T grown on BSG, reaching 518.2 U/ml, a value 8.4- and 4.7-fold higher than those achieved on xylan and D-xylose, respectively. The xylanase activities were characterized in relation to pH and temperature with optima at 4.5 and 50 °C, respectively. The extracts from both M. antarcticus PYCC 5048 T and M. aphidis PYCC 5535 T were used in xylan hydrolysis, producing D-xylose as the major end product (0.43 and 0.34-0.47 g D-xylose /g xylan , respectively, at 50 °C) and relatively low or no xylobiose accumulation (from no detection to 0.12 g D-xylobiose /g xylan at 50 °C).

OriginalsprogEngelsk
TidsskriftNew Biotechnology
Vol/bind49
Sider (fra-til)137-143
Antal sider7
ISSN1871-6784
DOI
StatusUdgivet - 25 mar. 2019

Fingerprint

Breweries
Xylans
Xylose
Enzymes
Substrates
Hydrolysis
Paper and pulp industry
Complex Mixtures
Yeast
Industrial applications
Industry
Yeasts
Costs and Cost Analysis
Food
Temperature

Citer dette

Faria, Nuno Torres ; Marques, Susana ; Ferreira, Frederico Castelo ; Fonseca, César. / Production of xylanolytic enzymes by Moesziomyces spp. using xylose, xylan and brewery's spent grain as substrates. I: New Biotechnology. 2019 ; Bind 49. s. 137-143.
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title = "Production of xylanolytic enzymes by Moesziomyces spp. using xylose, xylan and brewery's spent grain as substrates",
abstract = "Xylanases play a crucial role in the hydrolysis of xylan-rich hemicelluloses and have wide industrial applications in the fuel, food, feed and pulp and paper industries. The production of these enzymes at low cost is of paramount importance for their commercial deployment. Moesziomyces antarcticus PYCC 5048 T and M. aphidis PYCC 5535 T were screened for their ability to produce xylanolytic enzymes when grown on D-xylose, xylan (beechwood) and brewery's spent grain (BSG). The extracellular crude extracts produced were characterized and tested in xylan hydrolysis. The yeasts produced xylanolytic enzymes without cellulolytic activity on all the substrates tested. The highest xylanase volumetric activity was obtained with M. aphidis PYCC 5535 T grown on BSG, reaching 518.2 U/ml, a value 8.4- and 4.7-fold higher than those achieved on xylan and D-xylose, respectively. The xylanase activities were characterized in relation to pH and temperature with optima at 4.5 and 50 °C, respectively. The extracts from both M. antarcticus PYCC 5048 T and M. aphidis PYCC 5535 T were used in xylan hydrolysis, producing D-xylose as the major end product (0.43 and 0.34-0.47 g D-xylose /g xylan , respectively, at 50 °C) and relatively low or no xylobiose accumulation (from no detection to 0.12 g D-xylobiose /g xylan at 50 °C).",
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author = "Faria, {Nuno Torres} and Susana Marques and Ferreira, {Frederico Castelo} and C{\'e}sar Fonseca",
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Production of xylanolytic enzymes by Moesziomyces spp. using xylose, xylan and brewery's spent grain as substrates. / Faria, Nuno Torres; Marques, Susana; Ferreira, Frederico Castelo; Fonseca, César.

I: New Biotechnology, Bind 49, 25.03.2019, s. 137-143.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Production of xylanolytic enzymes by Moesziomyces spp. using xylose, xylan and brewery's spent grain as substrates

AU - Faria, Nuno Torres

AU - Marques, Susana

AU - Ferreira, Frederico Castelo

AU - Fonseca, César

N1 - Copyright © 2018. Published by Elsevier B.V.

PY - 2019/3/25

Y1 - 2019/3/25

N2 - Xylanases play a crucial role in the hydrolysis of xylan-rich hemicelluloses and have wide industrial applications in the fuel, food, feed and pulp and paper industries. The production of these enzymes at low cost is of paramount importance for their commercial deployment. Moesziomyces antarcticus PYCC 5048 T and M. aphidis PYCC 5535 T were screened for their ability to produce xylanolytic enzymes when grown on D-xylose, xylan (beechwood) and brewery's spent grain (BSG). The extracellular crude extracts produced were characterized and tested in xylan hydrolysis. The yeasts produced xylanolytic enzymes without cellulolytic activity on all the substrates tested. The highest xylanase volumetric activity was obtained with M. aphidis PYCC 5535 T grown on BSG, reaching 518.2 U/ml, a value 8.4- and 4.7-fold higher than those achieved on xylan and D-xylose, respectively. The xylanase activities were characterized in relation to pH and temperature with optima at 4.5 and 50 °C, respectively. The extracts from both M. antarcticus PYCC 5048 T and M. aphidis PYCC 5535 T were used in xylan hydrolysis, producing D-xylose as the major end product (0.43 and 0.34-0.47 g D-xylose /g xylan , respectively, at 50 °C) and relatively low or no xylobiose accumulation (from no detection to 0.12 g D-xylobiose /g xylan at 50 °C).

AB - Xylanases play a crucial role in the hydrolysis of xylan-rich hemicelluloses and have wide industrial applications in the fuel, food, feed and pulp and paper industries. The production of these enzymes at low cost is of paramount importance for their commercial deployment. Moesziomyces antarcticus PYCC 5048 T and M. aphidis PYCC 5535 T were screened for their ability to produce xylanolytic enzymes when grown on D-xylose, xylan (beechwood) and brewery's spent grain (BSG). The extracellular crude extracts produced were characterized and tested in xylan hydrolysis. The yeasts produced xylanolytic enzymes without cellulolytic activity on all the substrates tested. The highest xylanase volumetric activity was obtained with M. aphidis PYCC 5535 T grown on BSG, reaching 518.2 U/ml, a value 8.4- and 4.7-fold higher than those achieved on xylan and D-xylose, respectively. The xylanase activities were characterized in relation to pH and temperature with optima at 4.5 and 50 °C, respectively. The extracts from both M. antarcticus PYCC 5048 T and M. aphidis PYCC 5535 T were used in xylan hydrolysis, producing D-xylose as the major end product (0.43 and 0.34-0.47 g D-xylose /g xylan , respectively, at 50 °C) and relatively low or no xylobiose accumulation (from no detection to 0.12 g D-xylobiose /g xylan at 50 °C).

KW - Brewery's spent grain

KW - Enzymatic hydrolysis

KW - Moesziomyces spp.

KW - Xylan

KW - Xylanases

KW - Carbon/pharmacology

KW - Temperature

KW - Xylans/metabolism

KW - Industrial Waste/analysis

KW - Hydrolysis

KW - Xylose/metabolism

KW - Time Factors

KW - Basidiomycota/enzymology

KW - Endo-1,4-beta Xylanases/metabolism

KW - Hydrogen-Ion Concentration

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U2 - 10.1016/j.nbt.2018.11.001

DO - 10.1016/j.nbt.2018.11.001

M3 - Journal article

C2 - 30423436

AN - SCOPUS:85056597819

VL - 49

SP - 137

EP - 143

JO - New Biotechnology

JF - New Biotechnology

SN - 1871-6784

ER -