Proteins involved in attack and defence in Zygomycete-aphid interactions

Morten Nedergaard Grell, Annette Bruun Jensen, Lene Lange

Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskning

Resumé

Fungi within the order Entomophthorales (Zygomycota) are obligate biotrophic pathogens of insects. Initially, the fungi penetrate the cuticle of their host, where after they grow with a protoplast-like morphology within the host, slowly degrading and eventually killing it. We have performed a secretome study of field collected grain aphids (Sitobion avenae) in early stages of infection with entomophthoralean fungi using the transposon assisted signal trapping (TAST) technology (Becker et al. 2004, J Microbiol Methods 57, 123-133), thereby discovering a series of secreted enzymes of the pathogens and defense related proteins of the host. Now, selected proteins are being produced in an expression host for further studies. The conservation of the fungal secreted proteins in the species dominating the collected material, namely Pandora neoaphidis, Entomophthora planchoniana and Conidiobolus obscurus, and additional related fungi are under investigation. We anticipate that our work will shed light on this highly specialized group of fungi that has attracted substantial attention as potential bio-control agents.
OriginalsprogEngelsk
TidsskriftFungal Genetics Reports
Vol/bind56 (Suppl)
Sider (fra-til)445
ISSN1941-4757
StatusUdgivet - 2009
BegivenhedThe 25th Fungal Genetics Conference at Asilomar - Pacific Grove, USA
Varighed: 17 mar. 200922 mar. 2009

Konference

KonferenceThe 25th Fungal Genetics Conference at Asilomar
LandUSA
ByPacific Grove
Periode17/03/200922/03/2009

Fingerprint

Aphidoidea
fungi
proteins
Conidiobolus obscurus
Erynia neoaphidis
Entomophthora
Zygomycota
Entomophthorales
Sitobion avenae
entomopathogens
transposons
protoplasts
biological control agents
trapping
pathogens
enzymes
infection
methodology

Citer dette

Grell, M. N., Jensen, A. B., & Lange, L. (2009). Proteins involved in attack and defence in Zygomycete-aphid interactions. Fungal Genetics Reports, 56 (Suppl), 445.
Grell, Morten Nedergaard ; Jensen, Annette Bruun ; Lange, Lene. / Proteins involved in attack and defence in Zygomycete-aphid interactions. I: Fungal Genetics Reports. 2009 ; Bind 56 (Suppl). s. 445.
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abstract = "Fungi within the order Entomophthorales (Zygomycota) are obligate biotrophic pathogens of insects. Initially, the fungi penetrate the cuticle of their host, where after they grow with a protoplast-like morphology within the host, slowly degrading and eventually killing it. We have performed a secretome study of field collected grain aphids (Sitobion avenae) in early stages of infection with entomophthoralean fungi using the transposon assisted signal trapping (TAST) technology (Becker et al. 2004, J Microbiol Methods 57, 123-133), thereby discovering a series of secreted enzymes of the pathogens and defense related proteins of the host. Now, selected proteins are being produced in an expression host for further studies. The conservation of the fungal secreted proteins in the species dominating the collected material, namely Pandora neoaphidis, Entomophthora planchoniana and Conidiobolus obscurus, and additional related fungi are under investigation. We anticipate that our work will shed light on this highly specialized group of fungi that has attracted substantial attention as potential bio-control agents.",
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Grell, MN, Jensen, AB & Lange, L 2009, 'Proteins involved in attack and defence in Zygomycete-aphid interactions', Fungal Genetics Reports, bind 56 (Suppl), s. 445.

Proteins involved in attack and defence in Zygomycete-aphid interactions. / Grell, Morten Nedergaard; Jensen, Annette Bruun; Lange, Lene.

I: Fungal Genetics Reports, Bind 56 (Suppl), 2009, s. 445.

Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskning

TY - ABST

T1 - Proteins involved in attack and defence in Zygomycete-aphid interactions

AU - Grell, Morten Nedergaard

AU - Jensen, Annette Bruun

AU - Lange, Lene

PY - 2009

Y1 - 2009

N2 - Fungi within the order Entomophthorales (Zygomycota) are obligate biotrophic pathogens of insects. Initially, the fungi penetrate the cuticle of their host, where after they grow with a protoplast-like morphology within the host, slowly degrading and eventually killing it. We have performed a secretome study of field collected grain aphids (Sitobion avenae) in early stages of infection with entomophthoralean fungi using the transposon assisted signal trapping (TAST) technology (Becker et al. 2004, J Microbiol Methods 57, 123-133), thereby discovering a series of secreted enzymes of the pathogens and defense related proteins of the host. Now, selected proteins are being produced in an expression host for further studies. The conservation of the fungal secreted proteins in the species dominating the collected material, namely Pandora neoaphidis, Entomophthora planchoniana and Conidiobolus obscurus, and additional related fungi are under investigation. We anticipate that our work will shed light on this highly specialized group of fungi that has attracted substantial attention as potential bio-control agents.

AB - Fungi within the order Entomophthorales (Zygomycota) are obligate biotrophic pathogens of insects. Initially, the fungi penetrate the cuticle of their host, where after they grow with a protoplast-like morphology within the host, slowly degrading and eventually killing it. We have performed a secretome study of field collected grain aphids (Sitobion avenae) in early stages of infection with entomophthoralean fungi using the transposon assisted signal trapping (TAST) technology (Becker et al. 2004, J Microbiol Methods 57, 123-133), thereby discovering a series of secreted enzymes of the pathogens and defense related proteins of the host. Now, selected proteins are being produced in an expression host for further studies. The conservation of the fungal secreted proteins in the species dominating the collected material, namely Pandora neoaphidis, Entomophthora planchoniana and Conidiobolus obscurus, and additional related fungi are under investigation. We anticipate that our work will shed light on this highly specialized group of fungi that has attracted substantial attention as potential bio-control agents.

KW - Gene discovery

KW - Parasitic interactions

KW - Fungus-host interactions

KW - Entomopathogens

KW - Extracellular proteins

M3 - Conference abstract in journal

VL - 56 (Suppl)

SP - 445

JO - Fungal Genetics Reports

JF - Fungal Genetics Reports

SN - 1941-4757

ER -