TY - JOUR
T1 - Quorum quenching probiotics modulated digestive enzymes activity, growth performance, gut microflora, haemato-biochemical parameters and resistance against Vibrio harveyi in Asian seabass (Lates calcarifer)
AU - Ghanei-Motlagh, Reza
AU - Mohammadian, Takavar
AU - Gharibi, Darioush
AU - Khosravi, Mohammad
AU - Mahmoudi, Esmaeil
AU - Zarea, Mojtaba
AU - El-Matbouli, Mansour
AU - Menanteau-Ledouble, Simon
N1 - Funding Information:
This research was financed by a grant from Shahid Chamran University of Ahvaz Research Council (Grant No. vC98.299 ). The funding body had no role in the design of the study or interpretation of data.
Funding Information:
This research was financed by a grant from Shahid Chamran University of Ahvaz Research Council (Grant No. vC98.299). The funding body had no role in the design of the study or interpretation of data.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/1/30
Y1 - 2021/1/30
N2 - The use of probiotics is a promising and increasingly common strategy to maintain a healthy farming environment in an eco-friendly and sustainable manner. The goal of this study was to investigate the possible effects of dietary supplementation with autochthonous quorum quenching (QQ) probiotics on growth, digestive enzymes activity, haemato-biochemical responses, gut microbial communities and resistance against Vibrio harveyi in Asian seabass. Asian seabass (75 ± 0.6 g and 15.5 ± 0.3 cm) were fed with basal diet (control groups) and basal diet containing 1 × 109 CFU per g Bacillus thuringiensis QQ1 or Bacillus cereus QQ2 for 6 weeks. Feeding with diets supplemented with QQ probiotics for 35 days (pre-infection period) resulted in a significant (p < 0.05) alteration of the growth parameters (RGR, FCR, SGR, PER, FER and FI) and digestive enzymes activity (α-amylase, total protease, lipase, trypsin, alkaline phosphatase, T/C and A/T). Hematocrit (Hct) and total leucocyte count (TLC) were significantly (p < 0.05) elevated in fish fed with QQ1. Respiratory burst activity (RBA) likewise significantly (p < 0.05) increased in the probiotic groups relative to the control groups. Moreover, a significant decrease (p < 0.05) in the level of serum total cholesterol was observed in fish fed with probiotic. QQ1 and QQ2 were able to colonize the host's intestine at high number after 5 weeks. Hct and RBA in QQ1 and QQ2, globulin in QQ2 and TLC in QQ1 were significantly (p < 0.05) promoted compared to the positive control (PC) after infection with Vibrio harveyi (day 42). Serum triglycerides, cholesterol, alkaline phosphatase (ALP), alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and lactate dehydrogenase (LDH) were significantly (p < 0.05) lower in the probiotic treatments than the PC group after infection. QQ probionts significantly (p < 0.05) improved the cumulative survival of Asian seabass against V. harveyi. According to these findings, we suggest that B. cereus QQ2 and particularly B. thuringiensis QQ1 have a high potential to enhance the performances of Asian seabass and therefore can be effectively applied for the control of V. harveyi.
AB - The use of probiotics is a promising and increasingly common strategy to maintain a healthy farming environment in an eco-friendly and sustainable manner. The goal of this study was to investigate the possible effects of dietary supplementation with autochthonous quorum quenching (QQ) probiotics on growth, digestive enzymes activity, haemato-biochemical responses, gut microbial communities and resistance against Vibrio harveyi in Asian seabass. Asian seabass (75 ± 0.6 g and 15.5 ± 0.3 cm) were fed with basal diet (control groups) and basal diet containing 1 × 109 CFU per g Bacillus thuringiensis QQ1 or Bacillus cereus QQ2 for 6 weeks. Feeding with diets supplemented with QQ probiotics for 35 days (pre-infection period) resulted in a significant (p < 0.05) alteration of the growth parameters (RGR, FCR, SGR, PER, FER and FI) and digestive enzymes activity (α-amylase, total protease, lipase, trypsin, alkaline phosphatase, T/C and A/T). Hematocrit (Hct) and total leucocyte count (TLC) were significantly (p < 0.05) elevated in fish fed with QQ1. Respiratory burst activity (RBA) likewise significantly (p < 0.05) increased in the probiotic groups relative to the control groups. Moreover, a significant decrease (p < 0.05) in the level of serum total cholesterol was observed in fish fed with probiotic. QQ1 and QQ2 were able to colonize the host's intestine at high number after 5 weeks. Hct and RBA in QQ1 and QQ2, globulin in QQ2 and TLC in QQ1 were significantly (p < 0.05) promoted compared to the positive control (PC) after infection with Vibrio harveyi (day 42). Serum triglycerides, cholesterol, alkaline phosphatase (ALP), alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and lactate dehydrogenase (LDH) were significantly (p < 0.05) lower in the probiotic treatments than the PC group after infection. QQ probionts significantly (p < 0.05) improved the cumulative survival of Asian seabass against V. harveyi. According to these findings, we suggest that B. cereus QQ2 and particularly B. thuringiensis QQ1 have a high potential to enhance the performances of Asian seabass and therefore can be effectively applied for the control of V. harveyi.
KW - Asian seabass
KW - Bacillus
KW - Performance
KW - Quorum quenching probiotic
KW - Survival
KW - Vibrio harveyi
UR - http://www.scopus.com/inward/record.url?scp=85089909458&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2020.735874
DO - 10.1016/j.aquaculture.2020.735874
M3 - Journal article
AN - SCOPUS:85089909458
SN - 0044-8486
VL - 531
JO - Aquaculture
JF - Aquaculture
M1 - 735874
ER -