Abstract
Rapid antibiotic susceptibility testing is in highly demand in health-care fields as antimicrobial resistant bacterial strains emerge and spread. Here we describe an optical screening system (oCelloScope), which based on time-lapse imaging of 96 bacteria-antibiotic combinations at a time, introduces real-time detection of bacterial growth and antimicrobial susceptibility, with imaging material to support the automatically generated graphs. Automated antibiotic susceptibility tests of a monoculture showed statistically significant antibiotic effect within 6 minutes and within 30 minutes in complex samples from pigs suffering from catheter associated urinary tract infections. The oCelloScope system provides a fast high-throughput screening method to detect bacterial susceptibility that may entail earlier diagnosis and introduction of appropriate targeted therapy and thus combat the threat from multidrug-resistant pathogenic bacteria. The oCelloScope system can be employed for a broad range of applications within bacteriology and may present new vistas as a point-of-care instrument in both clinical and veterinarian settings.
Originalsprog | Engelsk |
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Tidsskrift | Journal of Clinical Microbiology |
Vol/bind | 51 |
Udgave nummer | 7 |
Sider (fra-til) | 2047-2053 |
ISSN | 0095-1137 |
DOI | |
Status | Udgivet - 17 apr. 2013 |