Reliable methodology to determine biotransformation of PBAT in anaerobic conditions

Alba Trueba-Santiso; Reinhard Wimmer; Mathias Eskildsen; Juan Cubero-Cardoso; Juan M Lema; Jeppe Lund Nielsen

doi:10.1016/j.biortech.2025.132242

Reliable methodology to determine biotransformation of PBAT in anaerobic conditions

Alba Trueba-Santiso, Reinhard Wimmer, Mathias Eskildsen, Juan Cubero-Cardoso, Juan M Lema, Jeppe Lund Nielsen^*

^*Kontaktforfatter

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

3 Citationer (Scopus)

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Abstract

Biodegradable plastics can enhance food waste utilization in anaerobic digestion (AD) units, but their fate under thermophilic conditions remains unclear. Previous studies using methane production, calorimetry, or spectroscopic analyses often report inconsistent results. This study tracks the biotransformation of polybutylene adipate co-terephthalate (PBAT) in thermophilic AD (55 °C) using 1H NMR to quantify monomers. While 1,4-butanediol degraded quickly, adipic (AA) and terephthalic acid (TPA) accumulated over time. Monomer analysis estimated PBAT biotransformation at 11.1 ± 1.9 % (TPA) and 10.1 ± 2.3 % (AA). The core microbial community remained stable, indicating intrinsic hydrolytic capacities, which were stable despite TPA and AA accumulation. This workflow provides a robust methodology to evaluate the biotransformation of plastics.

Originalsprog	Engelsk
Artikelnummer	132242
Tidsskrift	Bioresource Technology
Vol/bind	424
Antal sider	8
ISSN	0960-8524
DOI	https://doi.org/10.1016/j.biortech.2025.132242
Status	Udgivet - maj 2025

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10.1016/j.biortech.2025.132242Licens: CC BY 4.0

Open Acces ArticleForlagets udgivne version, 3,79 MBLicens: CC BY 4.0

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@article{8134930dea2a4b4fafcc6c4e36942b8e,

title = "Reliable methodology to determine biotransformation of PBAT in anaerobic conditions",

abstract = "Biodegradable plastics can enhance food waste utilization in anaerobic digestion (AD) units, but their fate under thermophilic conditions remains unclear. Previous studies using methane production, calorimetry, or spectroscopic analyses often report inconsistent results. This study tracks the biotransformation of polybutylene adipate co-terephthalate (PBAT) in thermophilic AD (55 °C) using 1H NMR to quantify monomers. While 1,4-butanediol degraded quickly, adipic (AA) and terephthalic acid (TPA) accumulated over time. Monomer analysis estimated PBAT biotransformation at 11.1 ± 1.9 \% (TPA) and 10.1 ± 2.3 \% (AA). The core microbial community remained stable, indicating intrinsic hydrolytic capacities, which were stable despite TPA and AA accumulation. This workflow provides a robust methodology to evaluate the biotransformation of plastics. ",

keywords = "Anaerobic digestion, H NMR, Metagenomics, PBAT, Plastic, Thermophilic",

author = "Alba Trueba-Santiso and Reinhard Wimmer and Mathias Eskildsen and Juan Cubero-Cardoso and Lema, \{Juan M\} and Nielsen, \{Jeppe Lund\}",

year = "2025",

month = may,

doi = "10.1016/j.biortech.2025.132242",

language = "English",

volume = "424",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier",

}

TY - JOUR

T1 - Reliable methodology to determine biotransformation of PBAT in anaerobic conditions

AU - Trueba-Santiso, Alba

AU - Wimmer, Reinhard

AU - Eskildsen, Mathias

AU - Cubero-Cardoso, Juan

AU - Lema, Juan M

AU - Nielsen, Jeppe Lund

PY - 2025/5

Y1 - 2025/5

N2 - Biodegradable plastics can enhance food waste utilization in anaerobic digestion (AD) units, but their fate under thermophilic conditions remains unclear. Previous studies using methane production, calorimetry, or spectroscopic analyses often report inconsistent results. This study tracks the biotransformation of polybutylene adipate co-terephthalate (PBAT) in thermophilic AD (55 °C) using 1H NMR to quantify monomers. While 1,4-butanediol degraded quickly, adipic (AA) and terephthalic acid (TPA) accumulated over time. Monomer analysis estimated PBAT biotransformation at 11.1 ± 1.9 % (TPA) and 10.1 ± 2.3 % (AA). The core microbial community remained stable, indicating intrinsic hydrolytic capacities, which were stable despite TPA and AA accumulation. This workflow provides a robust methodology to evaluate the biotransformation of plastics.

AB - Biodegradable plastics can enhance food waste utilization in anaerobic digestion (AD) units, but their fate under thermophilic conditions remains unclear. Previous studies using methane production, calorimetry, or spectroscopic analyses often report inconsistent results. This study tracks the biotransformation of polybutylene adipate co-terephthalate (PBAT) in thermophilic AD (55 °C) using 1H NMR to quantify monomers. While 1,4-butanediol degraded quickly, adipic (AA) and terephthalic acid (TPA) accumulated over time. Monomer analysis estimated PBAT biotransformation at 11.1 ± 1.9 % (TPA) and 10.1 ± 2.3 % (AA). The core microbial community remained stable, indicating intrinsic hydrolytic capacities, which were stable despite TPA and AA accumulation. This workflow provides a robust methodology to evaluate the biotransformation of plastics.

KW - Anaerobic digestion

KW - H NMR

KW - Metagenomics

KW - PBAT

KW - Plastic

KW - Thermophilic

UR - https://www.scopus.com/pages/publications/85219497029

U2 - 10.1016/j.biortech.2025.132242

DO - 10.1016/j.biortech.2025.132242

M3 - Journal article

C2 - 39986631

SN - 0960-8524

VL - 424

JO - Bioresource Technology

JF - Bioresource Technology

M1 - 132242

ER -

Reliable methodology to determine biotransformation of PBAT in anaerobic conditions

Abstract

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