TY - JOUR
T1 - RN7SL1 may be translated under oncogenic conditions
AU - Hara, Tomoaki
AU - Meng, Sikun
AU - Tsuji, Yoshiko
AU - Arao, Yasuko
AU - Saito, Yoshiko
AU - Sato, Hiromichi
AU - Motooka, Daisuke
AU - Uchida, Shizuka
AU - Ishii, Hideshi
PY - 2024/3/19
Y1 - 2024/3/19
N2 - RN7SL1 (RNA component of signal recognition particle 7SL1), a component of the signal recognition particle, is a non-coding RNA possessing a small ORF (smORF). However, whether it is translated into peptides is unknown. Here, we generated the RN7SL1-Green Fluorescent Protein (GFP) gene, in which the smORF of RN7SL1 was replaced by GFP, introduced it into 293T cells, and observed cells emitting GFP fluorescence. Furthermore, RNA-seq of GFP-positive cells revealed that they were in an oncogenic state, suggesting that RN7SL1 smORF may be translated under special conditions.
AB - RN7SL1 (RNA component of signal recognition particle 7SL1), a component of the signal recognition particle, is a non-coding RNA possessing a small ORF (smORF). However, whether it is translated into peptides is unknown. Here, we generated the RN7SL1-Green Fluorescent Protein (GFP) gene, in which the smORF of RN7SL1 was replaced by GFP, introduced it into 293T cells, and observed cells emitting GFP fluorescence. Furthermore, RNA-seq of GFP-positive cells revealed that they were in an oncogenic state, suggesting that RN7SL1 smORF may be translated under special conditions.
KW - Green Fluorescent Proteins/genetics
KW - Peptides/metabolism
KW - Signal Recognition Particle/metabolism
KW - ncRNA
KW - smORF
KW - RN7SL1
UR - http://www.scopus.com/inward/record.url?scp=85187747052&partnerID=8YFLogxK
U2 - 10.1073/pnas.2312322121
DO - 10.1073/pnas.2312322121
M3 - Letter
C2 - 38478683
SN - 0027-8424
VL - 121
SP - e2312322121
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 12
M1 - e2312322121
ER -