Secretome discoveries of obligate biotrophic organisms

Lene Lange, Morten Nedergaard Grell

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

Abstract

Pythium oligandrum is an oomycete mycoparasite that holds a potential as biocontrol agent against various soil-borne, plant pathogenic oomycetes and fungi. A dual organism cDNA library was made from shake flask cultured hyphal biomass of the plant pathogenic Pythium ultimum, infected with and overgrown by Pythium oligandrum. The cDNA library was screened for secreted proteins, using the transposon assisted signal trapping technique (TAST, Becker et al., J. Microbial Methods, 2004, 57(1), 123-33). TAST allows for discovery of secreted proteins from organisms where limited or no sequence information is available. It also allows for discovery of novel enzymes where no activity assay is available. This represents the first example of using TAST for discovery of secreted pathogenesis related proteins and peptides of oomycete interactions. Details of the TAST screening technique (especially useful for studying host – pathogen interactions) will be presented. TAST screening led to the identification of a series of pathogenesis related proteins. The most prominent hits will be described, including a number of cellulases, a xylanase, a lipase, enzyme inhibitors, cellulose-binding elicitor lectins (CBELs) and elicitin-like proteins. Expression and characterization of selected proteins in yeast (Pichia pastoris) will be exemplified.

OriginalsprogEngelsk
Publikationsdato2008
Antal sider1
StatusUdgivet - 2008
Udgivet eksterntJa
BegivenhedInternational Congress of Mycology - Istanbul , Tyrkiet
Varighed: 5 aug. 20089 aug. 2008

Konference

KonferenceInternational Congress of Mycology
Land/OmrådeTyrkiet
ByIstanbul
Periode05/08/200809/08/2008

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