Secretome discovery reveals lignocellulose degradation capacity of the ectomycorrhizal fungus Paxillus involutus

Doris Roth, Francois Rineau, Peter B. Olsen, Tomas Johansson, Andrea Lages Lino Vala, Morten Nedergaard Grell, Anders Tunlid, Lene Lange

Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskning

Resumé

To improve our understanding of the role ectomycorrhizal fungi play in biomass conversion, we studied the transcriptome of P. involutus grown on glass beads in extract of soil organic matter. The mycelium was used for a cDNA library screened by Transposon-Assisted Signal Trapping (TAST*) for genes encoding secreted proteins. We identified 11 glycoside hydrolases (GH), none of them being cellulases of the GH families 6, 7 and 45, which constitute the well described enzymatic cellulose degradation system from numerous efficient cellulolytic fungi. In contrast, several predicted enzymes, namely a laccase and oxidoreductases possibly contribute to hydroxyl radical formation. The most abundant GH found was GH61, although typically described as accessory protein in the enzymatic cellulolytic apparatus. All in all, our results suggest that the cellulose degradation system of P. involutus resembles the brown rot fungi systems. In addition, GH61 apparently acts as accessory protein both in enzymatic and in radical-based cellulolysis. * Becker et al., J. Microbial Methods, 2004, 57(1), 123-33.
OriginalsprogEngelsk
TidsskriftFungal Genetics Reports
Vol/bind58 (Suppl)
Sider (fra-til)80
ISSN1941-4757
StatusUdgivet - 2011
BegivenhedThe 26th Fungal Genetics Conference at Asilomar -
Varighed: 15 mar. 201120 mar. 2011

Konference

KonferenceThe 26th Fungal Genetics Conference at Asilomar
Periode15/03/201120/03/2011

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Paxillus involutus
lignocellulose
hydrolases
glycosides
fungi
degradation
cellulose
brown-rot fungi
cellulases
proteins
laccase
oxidoreductases
gene targeting
hydroxyl radicals
transcriptome
cDNA libraries
transposons
mycelium
glass
soil organic matter

Citer dette

Roth, D., Rineau, F., Olsen, P. B., Johansson, T., Vala, A. L. L., Grell, M. N., ... Lange, L. (2011). Secretome discovery reveals lignocellulose degradation capacity of the ectomycorrhizal fungus Paxillus involutus. Fungal Genetics Reports, 58 (Suppl), 80.
Roth, Doris ; Rineau, Francois ; Olsen, Peter B. ; Johansson, Tomas ; Vala, Andrea Lages Lino ; Grell, Morten Nedergaard ; Tunlid, Anders ; Lange, Lene. / Secretome discovery reveals lignocellulose degradation capacity of the ectomycorrhizal fungus Paxillus involutus. I: Fungal Genetics Reports. 2011 ; Bind 58 (Suppl). s. 80.
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title = "Secretome discovery reveals lignocellulose degradation capacity of the ectomycorrhizal fungus Paxillus involutus",
abstract = "To improve our understanding of the role ectomycorrhizal fungi play in biomass conversion, we studied the transcriptome of P. involutus grown on glass beads in extract of soil organic matter. The mycelium was used for a cDNA library screened by Transposon-Assisted Signal Trapping (TAST*) for genes encoding secreted proteins. We identified 11 glycoside hydrolases (GH), none of them being cellulases of the GH families 6, 7 and 45, which constitute the well described enzymatic cellulose degradation system from numerous efficient cellulolytic fungi. In contrast, several predicted enzymes, namely a laccase and oxidoreductases possibly contribute to hydroxyl radical formation. The most abundant GH found was GH61, although typically described as accessory protein in the enzymatic cellulolytic apparatus. All in all, our results suggest that the cellulose degradation system of P. involutus resembles the brown rot fungi systems. In addition, GH61 apparently acts as accessory protein both in enzymatic and in radical-based cellulolysis. * Becker et al., J. Microbial Methods, 2004, 57(1), 123-33.",
author = "Doris Roth and Francois Rineau and Olsen, {Peter B.} and Tomas Johansson and Vala, {Andrea Lages Lino} and Grell, {Morten Nedergaard} and Anders Tunlid and Lene Lange",
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Roth, D, Rineau, F, Olsen, PB, Johansson, T, Vala, ALL, Grell, MN, Tunlid, A & Lange, L 2011, 'Secretome discovery reveals lignocellulose degradation capacity of the ectomycorrhizal fungus Paxillus involutus', Fungal Genetics Reports, bind 58 (Suppl), s. 80.

Secretome discovery reveals lignocellulose degradation capacity of the ectomycorrhizal fungus Paxillus involutus. / Roth, Doris; Rineau, Francois; Olsen, Peter B.; Johansson, Tomas; Vala, Andrea Lages Lino; Grell, Morten Nedergaard; Tunlid, Anders; Lange, Lene.

I: Fungal Genetics Reports, Bind 58 (Suppl), 2011, s. 80.

Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskning

TY - ABST

T1 - Secretome discovery reveals lignocellulose degradation capacity of the ectomycorrhizal fungus Paxillus involutus

AU - Roth, Doris

AU - Rineau, Francois

AU - Olsen, Peter B.

AU - Johansson, Tomas

AU - Vala, Andrea Lages Lino

AU - Grell, Morten Nedergaard

AU - Tunlid, Anders

AU - Lange, Lene

PY - 2011

Y1 - 2011

N2 - To improve our understanding of the role ectomycorrhizal fungi play in biomass conversion, we studied the transcriptome of P. involutus grown on glass beads in extract of soil organic matter. The mycelium was used for a cDNA library screened by Transposon-Assisted Signal Trapping (TAST*) for genes encoding secreted proteins. We identified 11 glycoside hydrolases (GH), none of them being cellulases of the GH families 6, 7 and 45, which constitute the well described enzymatic cellulose degradation system from numerous efficient cellulolytic fungi. In contrast, several predicted enzymes, namely a laccase and oxidoreductases possibly contribute to hydroxyl radical formation. The most abundant GH found was GH61, although typically described as accessory protein in the enzymatic cellulolytic apparatus. All in all, our results suggest that the cellulose degradation system of P. involutus resembles the brown rot fungi systems. In addition, GH61 apparently acts as accessory protein both in enzymatic and in radical-based cellulolysis. * Becker et al., J. Microbial Methods, 2004, 57(1), 123-33.

AB - To improve our understanding of the role ectomycorrhizal fungi play in biomass conversion, we studied the transcriptome of P. involutus grown on glass beads in extract of soil organic matter. The mycelium was used for a cDNA library screened by Transposon-Assisted Signal Trapping (TAST*) for genes encoding secreted proteins. We identified 11 glycoside hydrolases (GH), none of them being cellulases of the GH families 6, 7 and 45, which constitute the well described enzymatic cellulose degradation system from numerous efficient cellulolytic fungi. In contrast, several predicted enzymes, namely a laccase and oxidoreductases possibly contribute to hydroxyl radical formation. The most abundant GH found was GH61, although typically described as accessory protein in the enzymatic cellulolytic apparatus. All in all, our results suggest that the cellulose degradation system of P. involutus resembles the brown rot fungi systems. In addition, GH61 apparently acts as accessory protein both in enzymatic and in radical-based cellulolysis. * Becker et al., J. Microbial Methods, 2004, 57(1), 123-33.

M3 - Conference abstract in journal

VL - 58 (Suppl)

SP - 80

JO - Fungal Genetics Reports

JF - Fungal Genetics Reports

SN - 1941-4757

ER -

Roth D, Rineau F, Olsen PB, Johansson T, Vala ALL, Grell MN et al. Secretome discovery reveals lignocellulose degradation capacity of the ectomycorrhizal fungus Paxillus involutus. Fungal Genetics Reports. 2011;58 (Suppl):80.