SELECTION SYSTEM FOR PHAGEMIDS USING PROTEOLYTICALLY SENSITIVE HELPER PHAGE

Riechmann Lutz; Kristensen Peter; Jestin Jean-Luc; Winter Gregory Paul; Riechmann Lutz *; Kristensen Peter *; Jestin Jean-Luc *; Winter Gregory Paul *

SELECTION SYSTEM FOR PHAGEMIDS USING PROTEOLYTICALLY SENSITIVE HELPER PHAGE

Riechmann Lutz (Opfinder), Kristensen Peter (Opfinder), Jestin Jean-Luc (Opfinder), Winter Gregory Paul (Opfinder), Riechmann Lutz * (Opfinder), Kristensen Peter * (Opfinder), Jestin Jean-Luc * (Opfinder), Winter Gregory Paul * (Opfinder)

Publikation: Patent

Abstract

A method for effecting helper phage rescue of a phagemid comprising a fusion polypeptide to form progeny bacteriophage wherein

(a) the helper phage encodes a viral coat protein comprising a protease sensitive cleavage site (b) the phagemid comprises a fusion polypeptide (c) the progeny phage are exposed to a protease capable of cleaving the proteolytically cleavable site such that the cleavage of the protein derived from the helper phage impairs its ability to mediate infection and (d) the progeny are propagated by infection. The helper phage has the protease sensitive site within p3 coat protein and is the filamentous bacteriophage M13, fd or a related species.

Originalsprog	Engelsk
IPC	C40B 40/ 02 A I
Patentnummer	GB2379933
Land/Område	Danmark
Prioritetsdato	13/05/1999
Prioritetsnummer	GB20000026143
Status	Udgivet - 26 mar. 2003

AUB Link

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Citationsformater

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title = "SELECTION SYSTEM FOR PHAGEMIDS USING PROTEOLYTICALLY SENSITIVE HELPER PHAGE",

abstract = "A method for effecting helper phage rescue of a phagemid comprising a fusion polypeptide to form progeny bacteriophage wherein (a) the helper phage encodes a viral coat protein comprising a protease sensitive cleavage site (b) the phagemid comprises a fusion polypeptide (c) the progeny phage are exposed to a protease capable of cleaving the proteolytically cleavable site such that the cleavage of the protein derived from the helper phage impairs its ability to mediate infection and (d) the progeny are propagated by infection. The helper phage has the protease sensitive site within p3 coat protein and is the filamentous bacteriophage M13, fd or a related species.",

author = "Riechmann Lutz and Kristensen Peter and Jestin Jean-Luc and {Gregory Paul}, Winter and {Lutz *}, Riechmann and {Peter *}, Kristensen and {Jean-Luc *}, Jestin and {Gregory Paul *}, Winter",

year = "2003",

month = mar,

day = "26",

language = "English",

type = "Patent",

note = "GB2379933; C40B 40/ 02 A I",

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TY - PAT

T1 - SELECTION SYSTEM FOR PHAGEMIDS USING PROTEOLYTICALLY SENSITIVE HELPER PHAGE

AU - Lutz, Riechmann

AU - Peter, Kristensen

AU - Jean-Luc, Jestin

AU - Gregory Paul, Winter

AU - Lutz , Riechmann

AU - Peter , Kristensen

AU - Jean-Luc , Jestin

AU - Gregory Paul , Winter

PY - 2003/3/26

Y1 - 2003/3/26

N2 - A method for effecting helper phage rescue of a phagemid comprising a fusion polypeptide to form progeny bacteriophage wherein (a) the helper phage encodes a viral coat protein comprising a protease sensitive cleavage site (b) the phagemid comprises a fusion polypeptide (c) the progeny phage are exposed to a protease capable of cleaving the proteolytically cleavable site such that the cleavage of the protein derived from the helper phage impairs its ability to mediate infection and (d) the progeny are propagated by infection. The helper phage has the protease sensitive site within p3 coat protein and is the filamentous bacteriophage M13, fd or a related species.

AB - A method for effecting helper phage rescue of a phagemid comprising a fusion polypeptide to form progeny bacteriophage wherein (a) the helper phage encodes a viral coat protein comprising a protease sensitive cleavage site (b) the phagemid comprises a fusion polypeptide (c) the progeny phage are exposed to a protease capable of cleaving the proteolytically cleavable site such that the cleavage of the protein derived from the helper phage impairs its ability to mediate infection and (d) the progeny are propagated by infection. The helper phage has the protease sensitive site within p3 coat protein and is the filamentous bacteriophage M13, fd or a related species.

M3 - Patent

M1 - GB2379933

ER -