TY - JOUR
T1 - Serum concentrations of matrix metalloproteinase-9, tissue inhibitor of matrix metalloproteinase-1 and alpha2-macroglobulin in healthy subjects after supplementation with different doses of marine n-3 fatty acids
AU - Vogt, Jes
AU - Andersen, Vibeke Ladefoged
AU - Andreasen, A
AU - Obel, T
AU - Christensen, Jeppe Hagstrup
AU - Schmidt, Erik Berg
PY - 2010
Y1 - 2010
N2 - Objective: Extracellular matrix modification by matrix metalloproteinase—9 (MMP—9), tissue inhibitor of matrix metalloproteinases—1 (TIMP—1) and α2—macroglobulin may affect the stability of atherosclerotic plaques. Marine n—3 polyunsaturated fatty acids (n—3 PUFA) may protect against plaque rupture. The aim was to investigate the effect of marine n—3 PUFA supplementation on serum levels of MMP—9, TIMP—1, and α2—macroglobulin. Methods: Healthy volunteers were randomized to receive capsules contributing either 6.6 g marine n—3 PUFA/day, 2.0 g marine n—3 PUFA/day or 6.6 g of olive oil (control). Serum MMP—9, TIMP—1 and α2—macroglobulin was measured at baseline and after 12 weeks of supplementation. One way ANOVA or Kruskal—Wallis test was used to compare groups. Results: 60 healthy volunteers were enrolled and no subjects dropped out of the 12 week study. There were no statistically significant changes in serum levels of MMP—9, TIMP—1, and α2—macroglobulin in any of the three treatment groups (P=0.85, P=0.23 and P=0.87, respectively). Conclusion: Supplementation with marine n—3 PUFA had no effect on serum levels of MMP—9, TIMP—1 and α2—macroglobulin in healthy subjects. The possible protection offered by marine n—3 PUFA against plaque rupture is therefore unlikely to be mediated through a change in serum levels of MMP—9, TIMP—1 and α2—macroglobulin.
AB - Objective: Extracellular matrix modification by matrix metalloproteinase—9 (MMP—9), tissue inhibitor of matrix metalloproteinases—1 (TIMP—1) and α2—macroglobulin may affect the stability of atherosclerotic plaques. Marine n—3 polyunsaturated fatty acids (n—3 PUFA) may protect against plaque rupture. The aim was to investigate the effect of marine n—3 PUFA supplementation on serum levels of MMP—9, TIMP—1, and α2—macroglobulin. Methods: Healthy volunteers were randomized to receive capsules contributing either 6.6 g marine n—3 PUFA/day, 2.0 g marine n—3 PUFA/day or 6.6 g of olive oil (control). Serum MMP—9, TIMP—1 and α2—macroglobulin was measured at baseline and after 12 weeks of supplementation. One way ANOVA or Kruskal—Wallis test was used to compare groups. Results: 60 healthy volunteers were enrolled and no subjects dropped out of the 12 week study. There were no statistically significant changes in serum levels of MMP—9, TIMP—1, and α2—macroglobulin in any of the three treatment groups (P=0.85, P=0.23 and P=0.87, respectively). Conclusion: Supplementation with marine n—3 PUFA had no effect on serum levels of MMP—9, TIMP—1 and α2—macroglobulin in healthy subjects. The possible protection offered by marine n—3 PUFA against plaque rupture is therefore unlikely to be mediated through a change in serum levels of MMP—9, TIMP—1 and α2—macroglobulin.
M3 - Journal article
SN - 0145-5680
VL - 56
SP - 102
EP - 109
JO - Cellular and Molecular Biology
JF - Cellular and Molecular Biology
ER -