TY - JOUR
T1 - Serum Lipoprotein Profiling by NMR Spectroscopy Reveals Alterations in HDL-1 and HDL-2 Apo-A2 Subfractions in Alzheimer's Disease
AU - Mortensen, Jonas Ellegaard
AU - Andreassen, Trygve
AU - Olsen, Dorte Aalund
AU - Vestergaard, Karsten
AU - Madsen, Jonna Skov
AU - Kristensen, Søren Risom
AU - Pedersen, Shona
PY - 2024/11/1
Y1 - 2024/11/1
N2 - Identifying biomarkers for Alzheimer's disease (AD) is crucial, due to its complex pathology, which involves dysfunction in lipid transport, contributing to neuroinflammation, synaptic loss, and impaired amyloid-β clearance. Nuclear magnetic resonance (NMR) is able to quantify and stratify lipoproteins. The study investigated lipoproteins in blood from AD patients, aiming to evaluate their diagnostic potential. Serum and plasma were collected from AD patients (n = 25) and healthy individuals (n = 25). We conducted a comprehensive lipoprotein profiling on serum samples using NMR spectroscopy, analysing 112 lipoprotein subfractions. In plasma, we measured unspecific markers of neuronal damage and AD hallmark proteins using single molecule array technology. Additionally, clinical data and cerebrospinal fluid biomarker levels were also collected to enrich our data. Our findings, after adjusting for age and sex differences, highlight significant alterations in two specific lipoproteins; high-density lipoprotein (HDL)-1 Apo-A2 (H1A2) and HDL-2 Apo-A2 (H2A2), both with area under the curve (AUC) values of 0.67, 95% confidence interval (CI) = 0.52-0.82). These results indicate that these lipoprotein subfractions may have potential as indicators of AD-related metabolic changes.
AB - Identifying biomarkers for Alzheimer's disease (AD) is crucial, due to its complex pathology, which involves dysfunction in lipid transport, contributing to neuroinflammation, synaptic loss, and impaired amyloid-β clearance. Nuclear magnetic resonance (NMR) is able to quantify and stratify lipoproteins. The study investigated lipoproteins in blood from AD patients, aiming to evaluate their diagnostic potential. Serum and plasma were collected from AD patients (n = 25) and healthy individuals (n = 25). We conducted a comprehensive lipoprotein profiling on serum samples using NMR spectroscopy, analysing 112 lipoprotein subfractions. In plasma, we measured unspecific markers of neuronal damage and AD hallmark proteins using single molecule array technology. Additionally, clinical data and cerebrospinal fluid biomarker levels were also collected to enrich our data. Our findings, after adjusting for age and sex differences, highlight significant alterations in two specific lipoproteins; high-density lipoprotein (HDL)-1 Apo-A2 (H1A2) and HDL-2 Apo-A2 (H2A2), both with area under the curve (AUC) values of 0.67, 95% confidence interval (CI) = 0.52-0.82). These results indicate that these lipoprotein subfractions may have potential as indicators of AD-related metabolic changes.
KW - Aged
KW - Aged, 80 and over
KW - Alzheimer Disease/blood
KW - Apolipoprotein A-II/blood
KW - Biomarkers/blood
KW - Female
KW - Humans
KW - Lipoproteins, HDL/blood
KW - Lipoproteins, HDL2/blood
KW - Magnetic Resonance Spectroscopy/methods
KW - Male
KW - Middle Aged
KW - Alzheimer’s disease
KW - biomarker
KW - blood
KW - nuclear magnetic resonance
KW - serum
KW - lipoproteins
UR - http://www.scopus.com/inward/record.url?scp=85208543380&partnerID=8YFLogxK
U2 - 10.3390/ijms252111701
DO - 10.3390/ijms252111701
M3 - Journal article
C2 - 39519253
SN - 1661-6596
VL - 25
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 21
M1 - 11701
ER -