Shedding light on novel bacteria in wastewater treatment plants: FISH probe design based on the MiDAS 3.1 16S rRNA ecosystem-specific database

Jannie Munk Kristensen; Lee-Ann Clegg; Francesca Petriglieri; Marta Anna Nierychlo; Morten Simonsen Dueholm; Per Halkjær Nielsen

Shedding light on novel bacteria in wastewater treatment plants: FISH probe design based on the MiDAS 3.1 16S rRNA ecosystem-specific database

Jannie Munk Kristensen, Lee-Ann Clegg, Francesca Petriglieri, Marta Anna Nierychlo, Morten Simonsen Dueholm, Per Halkjær Nielsen^*

^*Kontaktforfatter

Publikation: Konferencebidrag uden forlag/tidsskrift › Konferenceabstrakt til konference › Formidling

Abstract

Recent development of sequencing techniques has enabled us to create new ecosystem-specific full-length 16S rRNA gene reference databases, which gives a unique opportunity to design FISH probes for novel bacteria and revise existing FISH-probes. By using ecosystem-specific instead of universal large-scale databases, it is possible to design specific FISH probes for nearly all microbes in the given ecosystem, allowing visualization of novel microbial key players and combine these for studies of their ecophysiology. Here we have used a wastewater treatment-specific database to design FISH-probes for novel members of the phylum Acidobacteria, a hitherto overlooked but abundant group in activated sludge. Combination with Raman microspectroscopy provided functional information

Originalsprog	Engelsk
Publikationsdato	20 nov. 2019
Status	Udgivet - 20 nov. 2019
Begivenhed	8th IWA Microbial Ecology and Water Engineering Specialist Conference (MEWE2019) - Hiroshima, Japan Varighed: 17 nov. 2019 → 20 nov. 2019 http://mewe2019.org/

Konference

Konference	8th IWA Microbial Ecology and Water Engineering Specialist Conference (MEWE2019)
Land/Område	Japan
By	Hiroshima
Periode	17/11/2019 → 20/11/2019
Internetadresse	http://mewe2019.org/

AUB Link

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Citationsformater

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title = "Shedding light on novel bacteria in wastewater treatment plants: FISH probe design based on the MiDAS 3.1 16S rRNA ecosystem-specific database",

abstract = "Recent development of sequencing techniques has enabled us to create new ecosystem-specific full-length 16S rRNA gene reference databases, which gives a unique opportunity to design FISH probes for novel bacteria and revise existing FISH-probes. By using ecosystem-specific instead of universal large-scale databases, it is possible to design specific FISH probes for nearly all microbes in the given ecosystem, allowing visualization of novel microbial key players and combine these for studies of their ecophysiology. Here we have used a wastewater treatment-specific database to design FISH-probes for novel members of the phylum Acidobacteria, a hitherto overlooked but abundant group in activated sludge. Combination with Raman microspectroscopy provided functional information",

author = "Kristensen, {Jannie Munk} and Lee-Ann Clegg and Francesca Petriglieri and Nierychlo, {Marta Anna} and Dueholm, {Morten Simonsen} and Nielsen, {Per Halkj{\ae}r}",

year = "2019",

month = nov,

day = "20",

language = "English",

note = "8th IWA Microbial Ecology and Water Engineering Specialist Conference (MEWE2019) ; Conference date: 17-11-2019 Through 20-11-2019",

url = "http://mewe2019.org/",

}

Shedding light on novel bacteria in wastewater treatment plants: FISH probe design based on the MiDAS 3.1 16S rRNA ecosystem-specific database. / Kristensen, Jannie Munk ; Clegg, Lee-Ann ; Petriglieri, Francesca et al.
2019. Abstract fra 8th IWA Microbial Ecology and Water Engineering Specialist Conference (MEWE2019), Hiroshima, Japan.

Publikation: Konferencebidrag uden forlag/tidsskrift › Konferenceabstrakt til konference › Formidling

TY - ABST

T1 - Shedding light on novel bacteria in wastewater treatment plants: FISH probe design based on the MiDAS 3.1 16S rRNA ecosystem-specific database

AU - Kristensen, Jannie Munk

AU - Clegg, Lee-Ann

AU - Petriglieri, Francesca

AU - Nierychlo, Marta Anna

AU - Dueholm, Morten Simonsen

AU - Nielsen, Per Halkjær

PY - 2019/11/20

Y1 - 2019/11/20

N2 - Recent development of sequencing techniques has enabled us to create new ecosystem-specific full-length 16S rRNA gene reference databases, which gives a unique opportunity to design FISH probes for novel bacteria and revise existing FISH-probes. By using ecosystem-specific instead of universal large-scale databases, it is possible to design specific FISH probes for nearly all microbes in the given ecosystem, allowing visualization of novel microbial key players and combine these for studies of their ecophysiology. Here we have used a wastewater treatment-specific database to design FISH-probes for novel members of the phylum Acidobacteria, a hitherto overlooked but abundant group in activated sludge. Combination with Raman microspectroscopy provided functional information

AB - Recent development of sequencing techniques has enabled us to create new ecosystem-specific full-length 16S rRNA gene reference databases, which gives a unique opportunity to design FISH probes for novel bacteria and revise existing FISH-probes. By using ecosystem-specific instead of universal large-scale databases, it is possible to design specific FISH probes for nearly all microbes in the given ecosystem, allowing visualization of novel microbial key players and combine these for studies of their ecophysiology. Here we have used a wastewater treatment-specific database to design FISH-probes for novel members of the phylum Acidobacteria, a hitherto overlooked but abundant group in activated sludge. Combination with Raman microspectroscopy provided functional information

M3 - Conference abstract for conference

T2 - 8th IWA Microbial Ecology and Water Engineering Specialist Conference (MEWE2019)

Y2 - 17 November 2019 through 20 November 2019

ER -

Shedding light on novel bacteria in wastewater treatment plants: FISH probe design based on the MiDAS 3.1 16S rRNA ecosystem-specific database

Abstract

Konference

AUB Link

Fingeraftryk

Citationsformater