Abstrakt
Members of the Fusarium solani species complex are filamentous fungi that can act as pathogens to many
crops and animals. Although relevant, a robust molecular toolbox is missing for the investigation of gene
function and metabolism. In this chapter, we describe how Agrobacterium-mediated transformation can be
used to facilitate gene targeting. A flexible vector system, based on in vivo recombination in Saccharomyces
cerevisiae, is utilized to achieve overexpression and gene deletion of targeted biosynthetic genes in F. solani
f. sp. pisi.
crops and animals. Although relevant, a robust molecular toolbox is missing for the investigation of gene
function and metabolism. In this chapter, we describe how Agrobacterium-mediated transformation can be
used to facilitate gene targeting. A flexible vector system, based on in vivo recombination in Saccharomyces
cerevisiae, is utilized to achieve overexpression and gene deletion of targeted biosynthetic genes in F. solani
f. sp. pisi.
| Originalsprog | Engelsk |
|---|---|
| Titel | Engineering Natural Product Biosynthesis : Methods and Protocols |
| Redaktører | Elizabeth Skellam |
| Antal sider | 22 |
| Forlag | Springer |
| Publikationsdato | 7 maj 2022 |
| Sider | 93-114 |
| Kapitel | 6 |
| ISBN (Trykt) | 978-1-0716-2272-8 |
| ISBN (Elektronisk) | 978-1-0716-2273-5 |
| DOI | |
| Status | Udgivet - 7 maj 2022 |
| Navn | Methods in Molecular Biology |
|---|---|
| Vol/bind | 2489 |
| ISSN | 1064-3745 |