Targeted Genetic Engineering via Agrobacterium-Mediated Transformation in Fusarium solani

Mikkel Rank Nielsen*, Samba Eveline Kabemba Kaniki, Jens Laurids Sørensen

*Kontaktforfatter

Publikation: Bidrag til bog/antologi/rapport/konference proceedingBidrag til bog/antologiForskningpeer review

Abstract

Members of the Fusarium solani species complex are filamentous fungi that can act as pathogens to many
crops and animals. Although relevant, a robust molecular toolbox is missing for the investigation of gene
function and metabolism. In this chapter, we describe how Agrobacterium-mediated transformation can be
used to facilitate gene targeting. A flexible vector system, based on in vivo recombination in Saccharomyces
cerevisiae, is utilized to achieve overexpression and gene deletion of targeted biosynthetic genes in F. solani
f. sp. pisi.
OriginalsprogEngelsk
TitelEngineering Natural Product Biosynthesis : Methods and Protocols
RedaktørerElizabeth Skellam
Antal sider22
ForlagSpringer
Publikationsdato7 maj 2022
Sider93-114
Kapitel6
ISBN (Trykt)978-1-0716-2272-8
ISBN (Elektronisk)978-1-0716-2273-5
DOI
StatusUdgivet - 7 maj 2022
NavnMethods in Molecular Biology
Vol/bind2489
ISSN1064-3745

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