The DR6 protein from human herpesvirus-6B induces p53-independent cell cycle arrest in G2/M

Mariane H Schleimann, Søren Hoberg, Aida S Hansen, Bettina Bundgaard, Christoffer T Witt, Emil Kofod-Olsen, Per Höllsberg

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

10 Citationer (Scopus)

Abstract

HHV-6B infection inhibits cell proliferation in G2/M, but no protein has so far been recognized to exert this function. Here we identify the protein product of direct repeat 6, DR6, as an inhibitor of G2/M cell-cycle progression. Transfection of DR6 reduced the total number of cells compared with mock-transfected cells. Lentiviral transduction of DR6 inhibited host cell DNA synthesis in a p53-independent manner, and this inhibition was DR6 dose-dependent. A deletion of 66 amino acids from the N-terminal part of DR6 prevented efficient nuclear translocation and the ability to inhibit DNA synthesis. DR6-induced accumulation of cells in G2/M was accompanied by an enhanced expression of cyclin B1 that accumulated predominantly in the cytoplasm. Pull-down of cyclin B1 brought down pCdk1 with the inactivating phosphorylation at Tyr15. Together, DR6 delays cell cycle with an accumulation of cells in G2/M and thus might be involved in HHV-6B-induced cell-cycle arrest.

OriginalsprogEngelsk
TidsskriftVirology
Vol/bind452-453
Sider (fra-til)254-63
Antal sider10
ISSN0042-6822
DOI
StatusUdgivet - mar. 2014
Udgivet eksterntJa

Fingeraftryk

Dyk ned i forskningsemnerne om 'The DR6 protein from human herpesvirus-6B induces p53-independent cell cycle arrest in G2/M'. Sammen danner de et unikt fingeraftryk.

Citationsformater