The influence of glucosylation on the renaturation rate of T4 phage DNA

C. Christiansen; G. Christiansen; A. Leth Bak

doi:10.1016/0006-291X(73)90660-8

The influence of glucosylation on the renaturation rate of T4 phage DNA

C. Christiansen^*, G. Christiansen, A. Leth Bak

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Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

7 Citationer (Scopus)

Abstract

The kinetic complexity of T4 phage DNA with different degrees of glucosylation was determined by studies of DNA renaturation. It was found that this parameter decreased with decreasing degree of glucosylation, and that the kinetic complexity of non-glucosylated DNA was in agreement with the known genome size of T4 phage. This observation indicates that no significant correction for differences in GC content is necessary in the determination of genome sizes from renaturation data.

Originalsprog	Engelsk
Tidsskrift	Biochemical and Biophysical Research Communications
Vol/bind	52
Udgave nummer	4
Sider (fra-til)	1426-1433
Antal sider	8
ISSN	0006-291X
DOI	https://doi.org/10.1016/0006-291X(73)90660-8
Status	Udgivet - 19 jun. 1973
Udgivet eksternt	Ja

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Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

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abstract = "The kinetic complexity of T4 phage DNA with different degrees of glucosylation was determined by studies of DNA renaturation. It was found that this parameter decreased with decreasing degree of glucosylation, and that the kinetic complexity of non-glucosylated DNA was in agreement with the known genome size of T4 phage. This observation indicates that no significant correction for differences in GC content is necessary in the determination of genome sizes from renaturation data.",

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AU - Christiansen, G.

AU - Bak, A. Leth

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Y1 - 1973/6/19

N2 - The kinetic complexity of T4 phage DNA with different degrees of glucosylation was determined by studies of DNA renaturation. It was found that this parameter decreased with decreasing degree of glucosylation, and that the kinetic complexity of non-glucosylated DNA was in agreement with the known genome size of T4 phage. This observation indicates that no significant correction for differences in GC content is necessary in the determination of genome sizes from renaturation data.

AB - The kinetic complexity of T4 phage DNA with different degrees of glucosylation was determined by studies of DNA renaturation. It was found that this parameter decreased with decreasing degree of glucosylation, and that the kinetic complexity of non-glucosylated DNA was in agreement with the known genome size of T4 phage. This observation indicates that no significant correction for differences in GC content is necessary in the determination of genome sizes from renaturation data.

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DO - 10.1016/0006-291X(73)90660-8

M3 - Journal article

C2 - 4577829

AN - SCOPUS:0015829978

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JO - Biochemical and Biophysical Research Communications

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ER -

The influence of glucosylation on the renaturation rate of T4 phage DNA

Abstract

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