TY - JOUR
T1 - The Role of Plasma Extracellular Vesicles in Remote Ischemic Conditioning and Exercise-Induced Ischemic Tolerance
AU - Gu, Tingting
AU - Just, Jesper
AU - Stenz, Katrine Tang
AU - Yan, Yan
AU - Sieljacks, Peter
AU - Wang, Jakob
AU - Groennebaek, Thomas Skjaerlund
AU - Jakobsgaard, Jesper Emil
AU - Rindom, Emil
AU - Herskind, Jon
AU - Gravholt, Anders
AU - Lassen, Thomas Ravn
AU - Jørgensen, Mathias
AU - Bæk, Rikke
AU - Gutiérrez-Jiménez, Eugenio
AU - Iversen, Nina Kerting
AU - Rasmussen, Peter Mondrup
AU - Nyengaard, Jens Randel
AU - Jørgensen, Malene Møller
AU - de Paoli, Frank
AU - Bøtker, Hans Erik
AU - Kjems, Jørgen
AU - Vissing, Kristian
AU - Drasbek, Kim Ryun
PY - 2022/3/19
Y1 - 2022/3/19
N2 - Ischemic conditioning and exercise have been suggested for protecting against brain ischemia-reperfusion injury. However, the endogenous protective mechanisms stimulated by these interventions remain unclear. Here, in a comprehensive translational study, we investigated the protective role of extracellular vesicles (EVs) released after remote ischemic conditioning (RIC), blood flow restricted resistance exercise (BFRRE), or high-load resistance exercise (HLRE). Blood samples were collected from human participants before and at serial time points after intervention. RIC and BFRRE plasma EVs released early after stimulation improved viability of endothelial cells subjected to oxygen-glucose deprivation. Furthermore, post-RIC EVs accumulated in the ischemic area of a stroke mouse model, and a mean decrease in infarct volume was observed for post-RIC EVs, although not reaching statistical significance. Thus, circulating EVs induced by RIC and BFRRE can mediate protection, but the in vivo and translational effects of conditioned EVs require further experimental verification.
AB - Ischemic conditioning and exercise have been suggested for protecting against brain ischemia-reperfusion injury. However, the endogenous protective mechanisms stimulated by these interventions remain unclear. Here, in a comprehensive translational study, we investigated the protective role of extracellular vesicles (EVs) released after remote ischemic conditioning (RIC), blood flow restricted resistance exercise (BFRRE), or high-load resistance exercise (HLRE). Blood samples were collected from human participants before and at serial time points after intervention. RIC and BFRRE plasma EVs released early after stimulation improved viability of endothelial cells subjected to oxygen-glucose deprivation. Furthermore, post-RIC EVs accumulated in the ischemic area of a stroke mouse model, and a mean decrease in infarct volume was observed for post-RIC EVs, although not reaching statistical significance. Thus, circulating EVs induced by RIC and BFRRE can mediate protection, but the in vivo and translational effects of conditioned EVs require further experimental verification.
KW - Angiogenesis
KW - Brain ischemia
KW - Cell viability
KW - Human brain vascular endothelial cells
KW - Infarction
KW - Plasma extracellular vesicles
KW - Remote ischemic conditioning
KW - Resistance exercise
KW - Stroke
UR - http://www.scopus.com/inward/record.url?scp=85126703859&partnerID=8YFLogxK
U2 - 10.3390/ijms23063334
DO - 10.3390/ijms23063334
M3 - Journal article
C2 - 35328755
SN - 1661-6596
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 6
M1 - 3334
ER -