The Sheaths of Methanospirillum Are Made of a New Type of Amyloid Protein

Line Friis Bakmann Christensen, Lonnie Maria Hansen, Kai Finster, Gunna Christiansen, Per Halkjær Nielsen, Daniel Erik Otzen, Morten Simonsen Dueholm

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Resumé

The genera Methanospirillum and Methanosaeta contain species of anaerobic archaea that grow and divide within proteinaceous tubular sheaths that protect them from environmental stressors. The sheaths of Methanosaeta thermophila PT are composed of the 60.9 kDa major sheath protein MspA. In this study we show that sheaths purified from Methanospirillum hungatei JF-1 are regularly striated tubular structures with amyloid-like properties similar to those of Methanosaeta thermophila PT. Depolymerizing the sheaths from M. hungatei JF-1 allowed us to identify a 40.6 kDa protein (WP_011449234.1) that shares 23% sequence similarity to MspA from M. thermophila PT (ABK14853.1), indicating that they might be distant homologs. The genome of M. hungatei JF-1 encodes six homologs of the identified MspA protein. Several homologs also exist in the related strains Methanospirillum stamsii (7 homologs, 28-66% sequence identity), Methanospirillum lacunae (15 homologs, 29-60% sequence identity) and Methanolinea tarda (2 homologs, 31% sequence identity). The MspA protein discovered here could accordingly represent a more widely found sheath protein than the MspA from M. thermophila PT, which currently has no homologs in the NCBI Reference Sequence database (RefSeq).
OriginalsprogEngelsk
Artikelnummer2729
TidsskriftFrontiers in Microbiology
Vol/bind9
Antal sider11
ISSN1664-302X
DOI
StatusUdgivet - 13 nov. 2018

Citer dette

Christensen, Line Friis Bakmann ; Hansen, Lonnie Maria ; Finster, Kai ; Christiansen, Gunna ; Nielsen, Per Halkjær ; Otzen, Daniel Erik ; Dueholm, Morten Simonsen. / The Sheaths of Methanospirillum Are Made of a New Type of Amyloid Protein. I: Frontiers in Microbiology. 2018 ; Bind 9.
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title = "The Sheaths of Methanospirillum Are Made of a New Type of Amyloid Protein",
abstract = "The genera Methanospirillum and Methanosaeta contain species of anaerobic archaea that grow and divide within proteinaceous tubular sheaths that protect them from environmental stressors. The sheaths of Methanosaeta thermophila PT are composed of the 60.9 kDa major sheath protein MspA. In this study we show that sheaths purified from Methanospirillum hungatei JF-1 are regularly striated tubular structures with amyloid-like properties similar to those of Methanosaeta thermophila PT. Depolymerizing the sheaths from M. hungatei JF-1 allowed us to identify a 40.6 kDa protein (WP_011449234.1) that shares 23{\%} sequence similarity to MspA from M. thermophila PT (ABK14853.1), indicating that they might be distant homologs. The genome of M. hungatei JF-1 encodes six homologs of the identified MspA protein. Several homologs also exist in the related strains Methanospirillum stamsii (7 homologs, 28-66{\%} sequence identity), Methanospirillum lacunae (15 homologs, 29-60{\%} sequence identity) and Methanolinea tarda (2 homologs, 31{\%} sequence identity). The MspA protein discovered here could accordingly represent a more widely found sheath protein than the MspA from M. thermophila PT, which currently has no homologs in the NCBI Reference Sequence database (RefSeq).",
author = "Christensen, {Line Friis Bakmann} and Hansen, {Lonnie Maria} and Kai Finster and Gunna Christiansen and Nielsen, {Per Halkj{\ae}r} and Otzen, {Daniel Erik} and Dueholm, {Morten Simonsen}",
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The Sheaths of Methanospirillum Are Made of a New Type of Amyloid Protein. / Christensen, Line Friis Bakmann; Hansen, Lonnie Maria; Finster, Kai; Christiansen, Gunna; Nielsen, Per Halkjær; Otzen, Daniel Erik; Dueholm, Morten Simonsen.

I: Frontiers in Microbiology, Bind 9, 2729, 13.11.2018.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - The Sheaths of Methanospirillum Are Made of a New Type of Amyloid Protein

AU - Christensen, Line Friis Bakmann

AU - Hansen, Lonnie Maria

AU - Finster, Kai

AU - Christiansen, Gunna

AU - Nielsen, Per Halkjær

AU - Otzen, Daniel Erik

AU - Dueholm, Morten Simonsen

PY - 2018/11/13

Y1 - 2018/11/13

N2 - The genera Methanospirillum and Methanosaeta contain species of anaerobic archaea that grow and divide within proteinaceous tubular sheaths that protect them from environmental stressors. The sheaths of Methanosaeta thermophila PT are composed of the 60.9 kDa major sheath protein MspA. In this study we show that sheaths purified from Methanospirillum hungatei JF-1 are regularly striated tubular structures with amyloid-like properties similar to those of Methanosaeta thermophila PT. Depolymerizing the sheaths from M. hungatei JF-1 allowed us to identify a 40.6 kDa protein (WP_011449234.1) that shares 23% sequence similarity to MspA from M. thermophila PT (ABK14853.1), indicating that they might be distant homologs. The genome of M. hungatei JF-1 encodes six homologs of the identified MspA protein. Several homologs also exist in the related strains Methanospirillum stamsii (7 homologs, 28-66% sequence identity), Methanospirillum lacunae (15 homologs, 29-60% sequence identity) and Methanolinea tarda (2 homologs, 31% sequence identity). The MspA protein discovered here could accordingly represent a more widely found sheath protein than the MspA from M. thermophila PT, which currently has no homologs in the NCBI Reference Sequence database (RefSeq).

AB - The genera Methanospirillum and Methanosaeta contain species of anaerobic archaea that grow and divide within proteinaceous tubular sheaths that protect them from environmental stressors. The sheaths of Methanosaeta thermophila PT are composed of the 60.9 kDa major sheath protein MspA. In this study we show that sheaths purified from Methanospirillum hungatei JF-1 are regularly striated tubular structures with amyloid-like properties similar to those of Methanosaeta thermophila PT. Depolymerizing the sheaths from M. hungatei JF-1 allowed us to identify a 40.6 kDa protein (WP_011449234.1) that shares 23% sequence similarity to MspA from M. thermophila PT (ABK14853.1), indicating that they might be distant homologs. The genome of M. hungatei JF-1 encodes six homologs of the identified MspA protein. Several homologs also exist in the related strains Methanospirillum stamsii (7 homologs, 28-66% sequence identity), Methanospirillum lacunae (15 homologs, 29-60% sequence identity) and Methanolinea tarda (2 homologs, 31% sequence identity). The MspA protein discovered here could accordingly represent a more widely found sheath protein than the MspA from M. thermophila PT, which currently has no homologs in the NCBI Reference Sequence database (RefSeq).

U2 - 10.3389/fmicb.2018.02729

DO - 10.3389/fmicb.2018.02729

M3 - Journal article

VL - 9

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 2729

ER -