Verticillium dahliae disease resistance and the regulatory pathway for maturity and tuberization in potato

Helen H. Tai, David De Koyer, Mads Sønderkær, Sanne Hedegaard, Martin Lague, Claudia Goyer, Lana Nolan, Charlotte Davidson, Kyle Gardner, Jonathan Neilson, Jamuna Risal Paudel, Agnes Murphy, Benoit Bizimungu, Hui Ying Wang, Xingyao Xiong, Dennis Halterman, Kåre Lehmann Nielsen

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Resumé

Verticillium dahliae Kleb. is a pathogenic fungus causing wilting, chlorosis and early dying in potato. Genetic mapping of resistance to V. dahliae was done using a diploid population of potato. The major quantitative trait locus (QTL) for Verticillium resistance was found on chromosome 5. The StCDF1 gene controlling earliness of maturity and tuberization was mapped within the interval. Another QTL on chromosome 9 co-localised with the Ve2 Verticillium wilt resistance gene marker. Epistasis analysis indicated that the loci on chromosomes 5 and 9 had a
highly significant interaction, and that StCDF1 functioned downstream of Ve2. The StCDF1alleles were sequenced and found to encode StCDF1.1 and StCDF1.3. Interaction between the Ve2 resistance allele and the StCDF1.3 was demonstrated, but not for StCDF1.1. Genome-wide expression QTL (eQTL) analysis was carried out and genes with eQTL at the StCDF1 and Ve2 loci were both found to have similar functions involving the chloroplast, including photosynthesis, which declines in both maturity and Verticillium wilt. Among the GO terms that were specific to genes with eQTL at the Ve2, but not the StCDF1 locus, were those associated with fungal defense. These results suggest that Ve2 controls fungal defense and reduces early dying in Verticillium wilt through affecting genetic pathway controlling tuberization timing.
OriginalsprogEngelsk
Artikelnummer170040
TidsskriftThe Plant Genome
Vol/bind11
Udgave nummer1
Sider (fra-til)1
Antal sider1.163
ISSN1940-3372
DOI
StatusUdgivet - 2018

Citer dette

Tai, Helen H. ; De Koyer, David ; Sønderkær, Mads ; Hedegaard, Sanne ; Lague, Martin ; Goyer, Claudia ; Nolan, Lana ; Davidson, Charlotte ; Gardner, Kyle ; Neilson, Jonathan ; Paudel, Jamuna Risal ; Murphy, Agnes ; Bizimungu, Benoit ; Wang, Hui Ying ; Xiong, Xingyao ; Halterman, Dennis ; Nielsen, Kåre Lehmann. / Verticillium dahliae disease resistance and the regulatory pathway for maturity and tuberization in potato. I: The Plant Genome. 2018 ; Bind 11, Nr. 1. s. 1.
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title = "Verticillium dahliae disease resistance and the regulatory pathway for maturity and tuberization in potato",
abstract = "Verticillium dahliae Kleb. is a pathogenic fungus causing wilting, chlorosis and early dying in potato. Genetic mapping of resistance to V. dahliae was done using a diploid population of potato. The major quantitative trait locus (QTL) for Verticillium resistance was found on chromosome 5. The StCDF1 gene controlling earliness of maturity and tuberization was mapped within the interval. Another QTL on chromosome 9 co-localised with the Ve2 Verticillium wilt resistance gene marker. Epistasis analysis indicated that the loci on chromosomes 5 and 9 had ahighly significant interaction, and that StCDF1 functioned downstream of Ve2. The StCDF1alleles were sequenced and found to encode StCDF1.1 and StCDF1.3. Interaction between the Ve2 resistance allele and the StCDF1.3 was demonstrated, but not for StCDF1.1. Genome-wide expression QTL (eQTL) analysis was carried out and genes with eQTL at the StCDF1 and Ve2 loci were both found to have similar functions involving the chloroplast, including photosynthesis, which declines in both maturity and Verticillium wilt. Among the GO terms that were specific to genes with eQTL at the Ve2, but not the StCDF1 locus, were those associated with fungal defense. These results suggest that Ve2 controls fungal defense and reduces early dying in Verticillium wilt through affecting genetic pathway controlling tuberization timing.",
author = "Tai, {Helen H.} and {De Koyer}, David and Mads S{\o}nderk{\ae}r and Sanne Hedegaard and Martin Lague and Claudia Goyer and Lana Nolan and Charlotte Davidson and Kyle Gardner and Jonathan Neilson and Paudel, {Jamuna Risal} and Agnes Murphy and Benoit Bizimungu and Wang, {Hui Ying} and Xingyao Xiong and Dennis Halterman and Nielsen, {K{\aa}re Lehmann}",
year = "2018",
doi = "10.3835/plantgenome2017.05.0040",
language = "English",
volume = "11",
pages = "1",
journal = "The Plant Genome",
issn = "1940-3372",
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Tai, HH, De Koyer, D, Sønderkær, M, Hedegaard, S, Lague, M, Goyer, C, Nolan, L, Davidson, C, Gardner, K, Neilson, J, Paudel, JR, Murphy, A, Bizimungu, B, Wang, HY, Xiong, X, Halterman, D & Nielsen, KL 2018, 'Verticillium dahliae disease resistance and the regulatory pathway for maturity and tuberization in potato', The Plant Genome, bind 11, nr. 1, 170040, s. 1. https://doi.org/10.3835/plantgenome2017.05.0040

Verticillium dahliae disease resistance and the regulatory pathway for maturity and tuberization in potato. / Tai, Helen H.; De Koyer, David ; Sønderkær, Mads; Hedegaard, Sanne; Lague, Martin ; Goyer, Claudia; Nolan, Lana; Davidson, Charlotte ; Gardner, Kyle; Neilson, Jonathan ; Paudel, Jamuna Risal; Murphy, Agnes; Bizimungu, Benoit; Wang, Hui Ying; Xiong, Xingyao; Halterman, Dennis; Nielsen, Kåre Lehmann.

I: The Plant Genome, Bind 11, Nr. 1, 170040, 2018, s. 1.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Verticillium dahliae disease resistance and the regulatory pathway for maturity and tuberization in potato

AU - Tai, Helen H.

AU - De Koyer, David

AU - Sønderkær, Mads

AU - Hedegaard, Sanne

AU - Lague, Martin

AU - Goyer, Claudia

AU - Nolan, Lana

AU - Davidson, Charlotte

AU - Gardner, Kyle

AU - Neilson, Jonathan

AU - Paudel, Jamuna Risal

AU - Murphy, Agnes

AU - Bizimungu, Benoit

AU - Wang, Hui Ying

AU - Xiong, Xingyao

AU - Halterman, Dennis

AU - Nielsen, Kåre Lehmann

PY - 2018

Y1 - 2018

N2 - Verticillium dahliae Kleb. is a pathogenic fungus causing wilting, chlorosis and early dying in potato. Genetic mapping of resistance to V. dahliae was done using a diploid population of potato. The major quantitative trait locus (QTL) for Verticillium resistance was found on chromosome 5. The StCDF1 gene controlling earliness of maturity and tuberization was mapped within the interval. Another QTL on chromosome 9 co-localised with the Ve2 Verticillium wilt resistance gene marker. Epistasis analysis indicated that the loci on chromosomes 5 and 9 had ahighly significant interaction, and that StCDF1 functioned downstream of Ve2. The StCDF1alleles were sequenced and found to encode StCDF1.1 and StCDF1.3. Interaction between the Ve2 resistance allele and the StCDF1.3 was demonstrated, but not for StCDF1.1. Genome-wide expression QTL (eQTL) analysis was carried out and genes with eQTL at the StCDF1 and Ve2 loci were both found to have similar functions involving the chloroplast, including photosynthesis, which declines in both maturity and Verticillium wilt. Among the GO terms that were specific to genes with eQTL at the Ve2, but not the StCDF1 locus, were those associated with fungal defense. These results suggest that Ve2 controls fungal defense and reduces early dying in Verticillium wilt through affecting genetic pathway controlling tuberization timing.

AB - Verticillium dahliae Kleb. is a pathogenic fungus causing wilting, chlorosis and early dying in potato. Genetic mapping of resistance to V. dahliae was done using a diploid population of potato. The major quantitative trait locus (QTL) for Verticillium resistance was found on chromosome 5. The StCDF1 gene controlling earliness of maturity and tuberization was mapped within the interval. Another QTL on chromosome 9 co-localised with the Ve2 Verticillium wilt resistance gene marker. Epistasis analysis indicated that the loci on chromosomes 5 and 9 had ahighly significant interaction, and that StCDF1 functioned downstream of Ve2. The StCDF1alleles were sequenced and found to encode StCDF1.1 and StCDF1.3. Interaction between the Ve2 resistance allele and the StCDF1.3 was demonstrated, but not for StCDF1.1. Genome-wide expression QTL (eQTL) analysis was carried out and genes with eQTL at the StCDF1 and Ve2 loci were both found to have similar functions involving the chloroplast, including photosynthesis, which declines in both maturity and Verticillium wilt. Among the GO terms that were specific to genes with eQTL at the Ve2, but not the StCDF1 locus, were those associated with fungal defense. These results suggest that Ve2 controls fungal defense and reduces early dying in Verticillium wilt through affecting genetic pathway controlling tuberization timing.

U2 - 10.3835/plantgenome2017.05.0040

DO - 10.3835/plantgenome2017.05.0040

M3 - Journal article

VL - 11

SP - 1

JO - The Plant Genome

JF - The Plant Genome

SN - 1940-3372

IS - 1

M1 - 170040

ER -