Virulence of a Klebsiella pneumoniae strain carrying the New Delhi metallo-beta-lactamase-1 (NDM-1)

Kurt Fuursted; Lone Schøler; Karen Dam; Martin S Bojer; Anette M Hammerum; Frederik Dagnæs-Hansen; Anders Olsen; Yousef Jasemian; Carsten Struve

doi:10.1016/j.micinf.2011.08.015

Virulence of a Klebsiella pneumoniae strain carrying the New Delhi metallo-beta-lactamase-1 (NDM-1)

Kurt Fuursted, Lone Schøler, Karen Dam, Martin S Bojer, Anette M Hammerum, Frederik Dagnæs-Hansen, Anders Olsen, Yousef Jasemian, Carsten Struve

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

32 Citationer (Scopus)

Abstract

The aim of the study was to compare and evaluate virulence in five strains of Klebsiella pneumoniae, including an isolate carrying New Delhi metallo-beta-lactamase-1 (NDM-1). In vivo virulence was assessed using a murine sepsis model and using the nematode Caenorhabditis elegans killing model, and in vitro virulence by assessing various virulence factors. The NDM-1 carrying K. pneumoniae isolate was the most virulent in the murine sepsis model but there was no clear cut correlation to in vitro virulence factors or killing in C. elegans. It is concluded that K. pneumoniae carrying NDM-1 have an intrinsic virulence potential, which in coexistence with its multiresistance could promote and partly explain its epidemiological success.

Originalsprog	Engelsk
Tidsskrift	Microbes and Infection
Vol/bind	14
Udgave nummer	2
Sider (fra-til)	155-8
Antal sider	4
ISSN	1286-4579
DOI	https://doi.org/10.1016/j.micinf.2011.08.015
Status	Udgivet - feb. 2012
Udgivet eksternt	Ja

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abstract = "The aim of the study was to compare and evaluate virulence in five strains of Klebsiella pneumoniae, including an isolate carrying New Delhi metallo-beta-lactamase-1 (NDM-1). In vivo virulence was assessed using a murine sepsis model and using the nematode Caenorhabditis elegans killing model, and in vitro virulence by assessing various virulence factors. The NDM-1 carrying K. pneumoniae isolate was the most virulent in the murine sepsis model but there was no clear cut correlation to in vitro virulence factors or killing in C. elegans. It is concluded that K. pneumoniae carrying NDM-1 have an intrinsic virulence potential, which in coexistence with its multiresistance could promote and partly explain its epidemiological success.",

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author = "Kurt Fuursted and Lone Sch{\o}ler and Karen Dam and Bojer, {Martin S} and Hammerum, {Anette M} and Frederik Dagn{\ae}s-Hansen and Anders Olsen and Yousef Jasemian and Carsten Struve",

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T1 - Virulence of a Klebsiella pneumoniae strain carrying the New Delhi metallo-beta-lactamase-1 (NDM-1)

AU - Fuursted, Kurt

AU - Schøler, Lone

AU - Dam, Karen

AU - Bojer, Martin S

AU - Hammerum, Anette M

AU - Dagnæs-Hansen, Frederik

AU - Olsen, Anders

AU - Jasemian, Yousef

AU - Struve, Carsten

PY - 2012/2

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N2 - The aim of the study was to compare and evaluate virulence in five strains of Klebsiella pneumoniae, including an isolate carrying New Delhi metallo-beta-lactamase-1 (NDM-1). In vivo virulence was assessed using a murine sepsis model and using the nematode Caenorhabditis elegans killing model, and in vitro virulence by assessing various virulence factors. The NDM-1 carrying K. pneumoniae isolate was the most virulent in the murine sepsis model but there was no clear cut correlation to in vitro virulence factors or killing in C. elegans. It is concluded that K. pneumoniae carrying NDM-1 have an intrinsic virulence potential, which in coexistence with its multiresistance could promote and partly explain its epidemiological success.

AB - The aim of the study was to compare and evaluate virulence in five strains of Klebsiella pneumoniae, including an isolate carrying New Delhi metallo-beta-lactamase-1 (NDM-1). In vivo virulence was assessed using a murine sepsis model and using the nematode Caenorhabditis elegans killing model, and in vitro virulence by assessing various virulence factors. The NDM-1 carrying K. pneumoniae isolate was the most virulent in the murine sepsis model but there was no clear cut correlation to in vitro virulence factors or killing in C. elegans. It is concluded that K. pneumoniae carrying NDM-1 have an intrinsic virulence potential, which in coexistence with its multiresistance could promote and partly explain its epidemiological success.

KW - Animals

KW - Anti-Bacterial Agents/pharmacology

KW - Biofilms/growth & development

KW - Body Temperature

KW - Caenorhabditis elegans/microbiology

KW - Drug Resistance, Multiple, Bacterial

KW - Female

KW - Humans

KW - Kaplan-Meier Estimate

KW - Klebsiella Infections/microbiology

KW - Klebsiella pneumoniae/enzymology

KW - Mice

KW - Mice, Inbred C57BL

KW - Microbial Sensitivity Tests

KW - Models, Animal

KW - Sepsis/microbiology

KW - Virulence/genetics

KW - beta-Lactamases/genetics

U2 - 10.1016/j.micinf.2011.08.015

DO - 10.1016/j.micinf.2011.08.015

M3 - Journal article

C2 - 21925284

SN - 1286-4579

VL - 14

SP - 155

EP - 158

JO - Microbes and Infection

JF - Microbes and Infection

IS - 2

ER -

Virulence of a Klebsiella pneumoniae strain carrying the New Delhi metallo-beta-lactamase-1 (NDM-1)

Abstract

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