Who needs neighbors? PKS8 is a Stand-Alone Gene in Fusarium graminearum responsible for production of Gibepyrones and Prolipyrone B

Klaus Ringsborg Westphal, Asmus Toftkær Muurmann, Iben Engell Paulsen, Kim Tanja Hejselbak Nørgaard, Marie Lund Overgaard, Sebastian Mølvang Dall, Trine Aalborg, Reinhard Wimmer, Jens Laurids Sørensen, Teis Esben Sondergaard*

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Resumé

Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.
OriginalsprogEngelsk
Artikelnummer2232
TidsskriftMolecules
Vol/bind23
Udgave nummer9
ISSN1420-3049
DOI
StatusUdgivet - 2 sep. 2018

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Fusarium
genes
Genes
sequencing
genome
cytochromes
products
Mixed Function Oxygenases
Biological Products
entry
Cytochrome P-450 Enzyme System
Genome
Therapeutics

Citer dette

Westphal, Klaus Ringsborg ; Muurmann, Asmus Toftkær ; Paulsen, Iben Engell ; Nørgaard, Kim Tanja Hejselbak ; Overgaard, Marie Lund ; Dall, Sebastian Mølvang ; Aalborg, Trine ; Wimmer, Reinhard ; Sørensen, Jens Laurids ; Sondergaard, Teis Esben. / Who needs neighbors? PKS8 is a Stand-Alone Gene in Fusarium graminearum responsible for production of Gibepyrones and Prolipyrone B. I: Molecules. 2018 ; Bind 23, Nr. 9.
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title = "Who needs neighbors? PKS8 is a Stand-Alone Gene in Fusarium graminearum responsible for production of Gibepyrones and Prolipyrone B",
abstract = "Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.",
keywords = "Fusarium, Gibepyrones, Polyketide synthases, Prolipyrone, Secondary metabolites",
author = "Westphal, {Klaus Ringsborg} and Muurmann, {Asmus Toftk{\ae}r} and Paulsen, {Iben Engell} and N{\o}rgaard, {Kim Tanja Hejselbak} and Overgaard, {Marie Lund} and Dall, {Sebastian M{\o}lvang} and Trine Aalborg and Reinhard Wimmer and S{\o}rensen, {Jens Laurids} and Sondergaard, {Teis Esben}",
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Who needs neighbors? PKS8 is a Stand-Alone Gene in Fusarium graminearum responsible for production of Gibepyrones and Prolipyrone B. / Westphal, Klaus Ringsborg; Muurmann, Asmus Toftkær; Paulsen, Iben Engell; Nørgaard, Kim Tanja Hejselbak; Overgaard, Marie Lund; Dall, Sebastian Mølvang; Aalborg, Trine; Wimmer, Reinhard; Sørensen, Jens Laurids; Sondergaard, Teis Esben.

I: Molecules, Bind 23, Nr. 9, 2232, 02.09.2018.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Who needs neighbors? PKS8 is a Stand-Alone Gene in Fusarium graminearum responsible for production of Gibepyrones and Prolipyrone B

AU - Westphal, Klaus Ringsborg

AU - Muurmann, Asmus Toftkær

AU - Paulsen, Iben Engell

AU - Nørgaard, Kim Tanja Hejselbak

AU - Overgaard, Marie Lund

AU - Dall, Sebastian Mølvang

AU - Aalborg, Trine

AU - Wimmer, Reinhard

AU - Sørensen, Jens Laurids

AU - Sondergaard, Teis Esben

PY - 2018/9/2

Y1 - 2018/9/2

N2 - Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.

AB - Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.

KW - Fusarium

KW - Gibepyrones

KW - Polyketide synthases

KW - Prolipyrone

KW - Secondary metabolites

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U2 - 10.3390/molecules23092232

DO - 10.3390/molecules23092232

M3 - Journal article

VL - 23

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 9

M1 - 2232

ER -