Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B

Klaus Ringsborg Westphal; Asmus Toftkær Muurmann; Iben Engell Paulsen; Kim Tanja Hejselbak Nørgaard; Marie Lund Overgaard; Sebastian Mølvang Dall; Trine Aalborg; Reinhard Wimmer; Jens Laurids Sørensen; Teis Esben Sondergaard

doi:10.3390/molecules23092232

Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B

Klaus Ringsborg Westphal, Asmus Toftkær Muurmann, Iben Engell Paulsen, Kim Tanja Hejselbak Nørgaard, Marie Lund Overgaard, Sebastian Mølvang Dall, Trine Aalborg, Reinhard Wimmer, Jens Laurids Sørensen, Teis Esben Sondergaard^*

^*Kontaktforfatter

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.

Originalsprog	Engelsk
Tidsskrift	Molecules
Vol/bind	23
Udgave nummer	2232
ISSN	1420-3049
DOI	https://doi.org/10.3390/molecules23092232
Status	Udgivet - 2 sep. 2018

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Westphal, K. R., Muurmann, A. T., Paulsen, I. E., Nørgaard, K. T. H., Overgaard, M. L., Mølvang Dall, S., Aalborg, T., Wimmer, R., Sørensen, J. L., & Sondergaard, T. E. (2018). Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B. Molecules, 23(2232). https://doi.org/10.3390/molecules23092232

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title = "Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B",

abstract = "Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.",

keywords = "Gibepyrones, Prolipyrone, Secondary metabolites, Polyketide synthases, Fusarium",

author = "Westphal, {Klaus Ringsborg} and Muurmann, {Asmus Toftk{\ae}r} and Paulsen, {Iben Engell} and N{\o}rgaard, {Kim Tanja Hejselbak} and Overgaard, {Marie Lund} and {M{\o}lvang Dall}, Sebastian and Trine Aalborg and Reinhard Wimmer and S{\o}rensen, {Jens Laurids} and Sondergaard, {Teis Esben}",

year = "2018",

month = sep,

day = "2",

doi = "10.3390/molecules23092232",

language = "English",

volume = "23",

journal = "Molecules",

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TY - JOUR

T1 - Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B

AU - Westphal, Klaus Ringsborg

AU - Muurmann, Asmus Toftkær

AU - Paulsen, Iben Engell

AU - Nørgaard, Kim Tanja Hejselbak

AU - Overgaard, Marie Lund

AU - Mølvang Dall, Sebastian

AU - Aalborg, Trine

AU - Wimmer, Reinhard

AU - Sørensen, Jens Laurids

AU - Sondergaard, Teis Esben

PY - 2018/9/2

Y1 - 2018/9/2

N2 - Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.

AB - Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.

KW - Gibepyrones

KW - Prolipyrone

KW - Secondary metabolites

KW - Polyketide synthases

KW - Fusarium

UR - http://dx.doi.org/10.3390/molecules23092232

U2 - 10.3390/molecules23092232

DO - 10.3390/molecules23092232

M3 - Journal article

SN - 1420-3049

VL - 23

JO - Molecules

JF - Molecules

IS - 2232

ER -

Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B

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