TY - JOUR
T1 - Whole genome sequencing of Aggregatibacter species isolated from human clinical specimens and description of Aggregatibacter kilianii sp. nov.
AU - Murra, May
AU - Lützen, Lisbeth
AU - Barut, Aynur
AU - Zbinden, Reinhard
AU - Lund, Marianne
AU - Villesen, Palle
AU - Nørskov-Lauritsen, Niels
N1 - Copyright © 2018 American Society for Microbiology.
PY - 2018/7
Y1 - 2018/7
N2 - Aggregatibacter species are commensal bacteria of human mucosal surfaces that are sometimes involved in serious invasive infections. During the investigation of strains cultured from various clinical specimens, we encountered a coherent group of 10 isolates that could not be allocated to any validly named species by phenotype, mass spectrometry, or partial 16S rRNA gene sequencing. Whole-genome sequencing revealed a phylogenetic cluster related to but separate from Aggregatibacter aphrophilus. The mean in silico DNA hybridization value for strains of the new cluster versus A. Aphrophilus was 56% (range, 53.7 to 58.0%), whereas the average nucleotide identity was 94.4% (range, 93.9 to 94.8%). The new cluster exhibited aggregative properties typical of the genus Aggregatibacter. Key phenotypic tests for discrimination of the new cluster from validly named Aggregatibacter species are alanine-phenylalanine-proline arylamidase, N-acetylglucosamine, and-galactosidase. The name Aggregatibacter kilianii is proposed, with PN528 (CCUG 70536T or DSM 105094T) as the type strain.
AB - Aggregatibacter species are commensal bacteria of human mucosal surfaces that are sometimes involved in serious invasive infections. During the investigation of strains cultured from various clinical specimens, we encountered a coherent group of 10 isolates that could not be allocated to any validly named species by phenotype, mass spectrometry, or partial 16S rRNA gene sequencing. Whole-genome sequencing revealed a phylogenetic cluster related to but separate from Aggregatibacter aphrophilus. The mean in silico DNA hybridization value for strains of the new cluster versus A. Aphrophilus was 56% (range, 53.7 to 58.0%), whereas the average nucleotide identity was 94.4% (range, 93.9 to 94.8%). The new cluster exhibited aggregative properties typical of the genus Aggregatibacter. Key phenotypic tests for discrimination of the new cluster from validly named Aggregatibacter species are alanine-phenylalanine-proline arylamidase, N-acetylglucosamine, and-galactosidase. The name Aggregatibacter kilianii is proposed, with PN528 (CCUG 70536T or DSM 105094T) as the type strain.
KW - Abscesses
KW - Average nucleotide identity
KW - HACEK
KW - Haemophilus
KW - Phylogeny
UR - http://www.scopus.com/inward/record.url?scp=85049171145&partnerID=8YFLogxK
U2 - 10.1128/JCM.00053-18
DO - 10.1128/JCM.00053-18
M3 - Journal article
C2 - 29695522
SN - 0095-1137
VL - 56
SP - 1
EP - 10
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 7
M1 - e00053-18
ER -